protease from bacillus subtilis
 
Notes:
Used as a food additive [EAFUS]
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CAS Number: 51931-23-8 
ECHA EC Number: 257-520-8
Category: enzyme preparations and microorganisms
 
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FDA Mainterm (SATF):51931-23-8 ; PROTEASE FROM BACILLUS SUBTILIS
FDA Regulation:
FDA PART 184 -- DIRECT FOOD SUBSTANCES AFFIRMED AS GENERALLY RECOGNIZED AS SAFE
Subpart B--Listing of Specific Substances Affirmed as GRAS
Sec. 184.1150 Bacterially-derived protease enzyme preparation.
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Physical Properties:
Food Chemicals Codex Listed: No
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Organoleptic Properties:
 
Odor and/or flavor descriptions from others (if found).
 
 
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Cosmetic Information:
None found
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Suppliers:
 None found
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Safety Information:
 
Hazards identification
 
Classification of the substance or mixture
GHS Classification in accordance with 29 CFR 1910 (OSHA HCS)
None found.
GHS Label elements, including precautionary statements
 
Pictogram
 
Hazard statement(s)
None found.
Precautionary statement(s)
None found.
Oral/Parenteral Toxicity:
Not determined
Dermal Toxicity:
Not determined
Inhalation Toxicity:
Not determined
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Safety in Use Information:
Category: enzyme preparations and microorganisms
Recommendation for protease from bacillus subtilis usage levels up to:
 not for fragrance use.
 
Recommendation for protease from bacillus subtilis flavor usage levels up to:
 not for flavor use.
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Safety References:
EPA ACToR: Toxicology Data
EPA Substance Registry Services (SRS): Registry
National Institute of Allergy and Infectious Diseases: Data
Chemidplus: 0051931238
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References:
Pubchem (sid): 135279513
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Other Information:
FDA Substances Added to Food (formerly EAFUS): View
HMDB (The Human Metabolome Database): Search
FooDB: FDB010151
FDA Listing of Food Additive Status: View
VCF-Online: VCF Volatile Compounds in Food
Wikipedia: View
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Potential Blenders and core components note
 
None Found
 
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Potential Uses:
None Found
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Occurrence (nature, food, other): note
 bacillus subtilis
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 protease
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Synonyms:
 protease from bacillus subtilis
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Articles:
PubMed: Potential probiotic attributes of a new strain of Bacillus coagulans CGMCC 9951 isolated from healthy piglet feces.
PubMed: Production and estimation of alkaline protease by immobilized Bacillus licheniformis isolated from poultry farm soil of 24 Parganas and its reusability.
PubMed: Screening of microorganisms from deep-sea mud for Antarctic krill (Euphausia superba) fermentation and evaluation of the bioactive compounds.
PubMed: Production and characterization of thermostable alkaline protease of B. Subtilis (ATCC 6633) from optimized solid state fermentation.
PubMed: Production of a water-soluble fertilizer containing amino acids by solid-state fermentation of soybean meal and evaluation of its efficacy on the rapeseed growth.
PubMed: Enhanced thermal stability and hydrolytic ability of Bacillus subtilis aminopeptidase by removing the thermal sensitive domain in the non-catalytic region.
PubMed: Preparation of the antithrombotic and antimicrobial coating through layer-by-layer self-assembly of nattokinase-nanosilver complex and polyethylenimine.
PubMed: Surface display of human growth hormone on Bacillus subtilis spores for oral administration.
PubMed: Controlled fermentation and preservation of UGBA -an indigenous Nigerian fermented food.
PubMed: Induction of apoptosis in human cancer cells by a Bacillus lipopeptide bacillomycin D.
PubMed: Characterization of a fibrinolytic enzyme secreted by Bacillus amyloliquefaciens CB1 and its gene cloning.
PubMed: Alkaline serine protease AprE plays an essential role in poly-γ-glutamate production during natto fermentation.
PubMed: Production of Enzymes from Agroindustrial Wastes by Biosurfactant-Producing Strains of Bacillus subtilis.
PubMed: Secretory expression of lacticin Q fused with SUMO in Bacillus subtilis.
PubMed: Co-production of surfactin and a novel bacteriocin by Bacillus subtilis subsp. subtilis H4 isolated from Bikalga, an African alkaline Hibiscus sabdariffa seed fermented condiment.
PubMed: Composition, characteristics, and in-vitro physiological effects of the water-soluble polysaccharides from Cassia seed.
PubMed: Over-expression, secretion, biochemical characterisation, and structure analysis of Bacillus subtilis aminopeptidase.
PubMed: Antioxidant and antimicrobial activity of xylan-chitooligomer-zinc complex.
PubMed: Detoxification and anti-nutrients reduction of Jatropha curcas seed cake by Bacillus fermentation.
PubMed: Strain screening, fermentation, separation, and encapsulation for production of nattokinase functional food.
PubMed: Thrombolytic effects of Douchi fibrinolytic enzyme from Bacillus subtilis LD-8547 in vitro and in vivo.
PubMed: Properties of Bac W42, a bacteriocin produced by Bacillus subtilis W42 isolated from Cheonggukjang.
PubMed: Inhibition of angiotensin I converting enzyme by subtilisin NAT (nattokinase) in natto, a Japanese traditional fermented food.
PubMed: Characterization of LysB4, an endolysin from the Bacillus cereus-infecting bacteriophage B4.
PubMed: Physicochemical Properties of Roasted Soybean Flour Bioconverted by Solid-State Fermentation Using Bacillus subtilis and Lactobacillus plantarum.
PubMed: Bacteriocin formation by dominant aerobic sporeformers isolated from traditional maari.
PubMed: Fibrinolytic enzyme from newly isolated marine bacterium Bacillus subtilis ICTF-1: media optimization, purification and characterization.
PubMed: Purification and characterization of a major fibrinolytic enzyme from Bacillus amyloliquefaciens MJ5-41 isolated from Meju.
PubMed: Extracellular production of cycloisomaltooligosaccharide glucanotransferase and cyclodextran by a protease-deficient Bacillus subtilis host-vector system.
PubMed: Fibrinolytic and antiplatelet aggregation properties of a recombinant Cheonggukjang kinase.
PubMed: A new method for multiple gene inactivations in Bacillus subtilis 168, producing a strain free of selectable markers.
PubMed: Disruption of the cell wall lytic enzyme CwlO affects the amount and molecular size of poly-γ-glutamic acid produced by Bacillus subtilis (natto).
PubMed: New insights into S2P signaling cascades: regulation, variation, and conservation.
PubMed: Application of exogenous enzymes to beef muscle of high and low-connective tissue.
PubMed: Construction of chromosomally located T7 expression system for production of heterologous secreted proteins in Bacillus subtilis.
PubMed: Bioproperties of potent nattokinase from Bacillus subtilis YJ1.
PubMed: Chelating and radical scavenging activities of soy protein hydrolysates prepared from microbial proteases and their effect on meat lipid peroxidation.
PubMed: A new antimicrobial peptide isolated from Oudneya africana seeds.
PubMed: Characterization of a 27 kDa fibrinolytic enzyme from Bacillus amyloliquefaciens CH51 isolated from cheonggukjang.
PubMed: Immune responses of Fenneropenaeus chinensis against white spot syndrome virus after oral delivery of VP28 using Bacillus subtilis as vehicles.
PubMed: Purification and characterization of nattokinase from Bacillus subtilis natto B-12.
PubMed: Improvement in the growth performance of white shrimp, Litopenaeus vannamei, by a protease-producing probiotic, Bacillus subtilis E20, from natto.
PubMed: Amyloid-degrading ability of nattokinase from Bacillus subtilis natto.
PubMed: The Lactobacillus plantarum ftsH gene is a novel member of the CtsR stress response regulon.
PubMed: A simple and cost-saving approach to optimize the production of subtilisin NAT by submerged cultivation of Bacillus subtilis natto.
PubMed: Neutrase immobilization on alginate-glutaraldehyde beads by covalent attachment.
PubMed: Construction of recombinant industrial Saccharomyces cerevisiae strain with bglS gene insertion into PEP4 locus by homologous recombination.
PubMed: New fermentation technique for complete digestion of soybean protein.
PubMed: Dimeric inhibitors of human salivary alpha-amylase from emmer (Triticum dicoccon Schrank) seeds.
PubMed: Preparation of whey protein hydrolysates using a single- and two-stage enzymatic membrane reactor and their immunological and antioxidant properties: characterization by multivariate data analysis.
PubMed: Antimicrobial activity of Bacillus subtilis and Bacillus pumilus during the fermentation of African locust bean (Parkia biglobosa) for Soumbala production.
PubMed: Recombinant eggshell ovocalyxin-32: expression, purification and biological activity of the glutathione S-transferase fusion protein.
PubMed: Comparison of the effects of acylation and amidation on the antimicrobial and antiviral properties of lactoferrin.
PubMed: Secretory expression of a heterologous nattokinase in Lactococcus lactis.
PubMed: Improved subtilisin YaB production in Bacillus subtilis using engineered synthetic expression control sequences.
PubMed: Supernatant of bacterial fermented soybean induces apoptosis of human hepatocellular carcinoma Hep 3B cells via activation of caspase 8 and mitochondria.
PubMed: In vitro fermentation studies for selection and evaluation of Bacillus strains as starter cultures for the production of okpehe, a traditional African fermented condiment.
PubMed: Characterization of Bacillus subtilis strains in Thua nao, a traditional fermented soybean food in northern Thailand.
PubMed: Purification and characterization of a halotolerant intracellular protease from Bacillus subtilis strain FP-133.
PubMed: Purification and characterization of a fibrinolytic enzyme of Bacillus subtilis DC33, isolated from Chinese traditional Douchi.
PubMed: Improvement of alpha-amylase production by modulation of ribosomal component protein S12 in Bacillus subtilis 168.
PubMed: A newly derived protein from Bacillus subtilis natto with both antithrombotic and fibrinolytic effects.
PubMed: The comparative ability of four isolates of Bacillus subtilis to ferment soybeans into dawadawa.
PubMed: Effect of boiling and roasting on the fermentation of soybeans into dawadawa (soy-dawadawa).
PubMed: Efficient system of artificial oil bodies for functional expression and purification of recombinant nattokinase in Escherichia coli.
PubMed: Growth and toxin production by Clostridium botulinum in steamed rice aseptically packed under modified atmosphere.
PubMed: The ywad gene from Bacillus subtilis encodes a double-zinc aminopeptidase.
PubMed: Nano-scale proteomics approach using two-dimensional fibrin zymography combined with fluorescent SYPRO ruby dye.
PubMed: Extracellular production of a functional soy cystatin by Bacillus subtilis.
PubMed: Isolation and purification of an extracellular protease from a new strain of Bacillus subtilis, viz.NCIM 2711.
PubMed: Isolation, characterization, and identification of bacterial contaminants in semifinal gelatin extracts.
PubMed: Cloning and expression of a fibrinolytic enzyme (subtilisin DFE) gene from Bacillus amyloliquefaciens DC-4 in Bacillus subtilis.
PubMed: Optimization of cultural conditions for thermostable beta-1,3-1,4-glucanase production by Bacillus subtilis ZJF-1A5.
PubMed: ClpE from Lactococcus lactis promotes repression of CtsR-dependent gene expression.
PubMed: Studies on the mechanism of the osmoresistance of spores of Bacillus subtilis.
PubMed: Reduction of protease activity in milk by continuous flow high-intensity pulsed electric field treatments.
PubMed: Study of the bacterial load in a gelatine production process focussed on Bacillus and related endosporeforming genera.
PubMed: Application potency of engineered G159 mutants on P1 substrate pocket of subtilisin YaB as improved meat tenderizers.
PubMed: Antimicrobial activity of foodborne Paenibacillus and Bacillus spp. against Clostridium botulinum.
PubMed: Antibacterial activity of licochalcone A against spore-forming bacteria.
PubMed: Scavenging of free radicals, antimicrobial, and cytotoxic activities of the Maillard reaction products of beta-lactoglobulin glycated with several sugars.
PubMed: Characterization of a keratinolytic serine protease from Bacillus subtilis KS-1.
PubMed: Formation of biogenic amines in raw milk Hispánico cheese manufactured with proteinases and different levels of starter culture.
PubMed: Improved growth and viability of lactobacilli in the presence of Bacillus subtilis (natto), catalase, or subtilisin.
PubMed: Improvement of the traditional processing and fermentation of African oil bean (Pentaclethra macrophylla Bentham) into a food snack--'ugba'.
PubMed: Potent fibrinolytic enzyme from a mutant of Bacillus subtilis IMR-NK1.
PubMed: An evaluation of the effect of Bacillus cells and Bacillus spores in association with cowpea granules as starter cultures for the fermentation of African oil bean (Pentaclethra macrophylla Bentham) to 'ugba'.
PubMed: Enhanced alkaline protease production in addition to alpha-amylase via constructing a Bacillus subtilis strain.
PubMed: ctsR of Lactococcus lactis encodes a negative regulator of clp gene expression.
PubMed: Regulation of stress response in Oenococcus oeni as a function of environmental changes and growth phase.
PubMed: Characteristics of a sporulation stimulating factor from Clostridium perfringens type A.
PubMed: Characteristics of the biologically active 35-kDa metalloprotease virulence factor from Listeria monocytogenes.
PubMed: Effect of added proteinases and level of starter culture on the formation of biogenic amines in raw milk Manchego cheese.
PubMed: Production and purification of protease from a Bacillus subtilis that can deproteinize crustacean wastes*
PubMed: Characterization and heterologous expression of the genes encoding enterocin a production, immunity, and regulation in Enterococcus faecium DPC1146.
PubMed: Fermentation of milk permeate by proteolytic bacteria for protease production.
PubMed: Digestion of staphylococcal enterotoxin by Bacillus natto.
PubMed: An evaluation of the microflora associated with fermented African oil bean (Pentaclethra macrophylla Bentham) seeds during ugba production.
PubMed: Selection of starter cultures for the production of kinema, a fermented soybean food of the Himalaya.
PubMed: Control of ammonia formation during Bacillus subtilis fermentation of legumes.
PubMed: Stunting syndrome in broilers: effect of age and exogenous amylase and protease on performance, development of the digestive tract, digestive enzyme activity, and apparent digestibility.
PubMed: Cloning and sequencing the degS-degU operon from an alkalophilic Bacillus brevis.
PubMed: Cloning, characterization, and inactivation of the Bacillus brevis lon gene.
PubMed: Cloning and expression of the lysostaphin gene in Bacillus subtilis and Lactobacillus casei.
PubMed: Heterologous gene expression in Lactococcus lactis subsp. lactis: synthesis, secretion, and processing of the Bacillus subtilis neutral protease.
PubMed: Conjugal Transfer of Plasmid-Encoded Determinants for Bacteriocin Production and Immunity in Lactobacillus acidophilus 88.
PubMed: Characterization of the inhibitory power of Lactobacillus of meat origin.
PubMed: A simple system for extending refrigerated, nonfrozen preservation of biological material using pressure.
PubMed: [Do aerobic sporers contain endotoxins which can be activated by trypsin digestion?].
PubMed: [Food hygienic studies of sulfite. 3. Effects of sulphus oxides on microbial hydrolytic enzymes and digestive enzymes].
PubMed: Symposium on microbial changes in foods. Application of microbial proteases to soybean and other materials to improve acceptability, especially through the formation of plastein.
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