alpha-amylase from bacillus stearothermophilus
 
Notes:
Used as a food additive [EAFUS]
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CAS Number: 98002-53-0 
Category: starch hydrolysis
 
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JECFA Food Additive: alpha-Amylase from Bacillus stearothermophilus
GSFA Codex: alpha-Amylase from Bacillus stearothermophilus (1100)
FDA Mainterm (SATF):98002-53-0 ; ALPHA-AMYLASE ENZYME PREPARATION FROM BACILLUS STEAROTHERMOPHILUS
FDA Regulation:
FDA PART 184 -- DIRECT FOOD SUBSTANCES AFFIRMED AS GENERALLY RECOGNIZED AS SAFE
Subpart B--Listing of Specific Substances Affirmed as GRAS
Sec. 184.1012 [b.alpha]-Amylase enzyme preparation from Bacillus stearothermophilus.
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Physical Properties:
Appearance: tan to dark brown powder to liquid (est)
Food Chemicals Codex Listed: No
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Organoleptic Properties:
 
Odor and/or flavor descriptions from others (if found).
 
 
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Cosmetic Information:
None found
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 None found
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Safety Information:
 
Hazards identification
 
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GHS Classification in accordance with 29 CFR 1910 (OSHA HCS)
None found.
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Pictogram
 
Hazard statement(s)
None found.
Precautionary statement(s)
None found.
Oral/Parenteral Toxicity:
Not determined
Dermal Toxicity:
Not determined
Inhalation Toxicity:
Not determined
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Safety in Use Information:
Category: starch hydrolysis
Recommendation for alpha-amylase from bacillus stearothermophilus usage levels up to:
 not for fragrance use.
 
Recommendation for alpha-amylase from bacillus stearothermophilus flavor usage levels up to:
 not for flavor use.
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Safety References:
European Food Safety Authority (EFSA) reference(s):
Explanatory Note for the Guidance of the Scientific Panel of Food Contact Material, Enzymes, Flavourings and Processing Aids (CEF) on the Submission of a Dossier on Food Enzymes
View page or View pdf
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EPA ACToR: Toxicology Data
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National Institute of Allergy and Infectious Diseases: Data
Chemidplus: 0098002530
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References:
Pubchem (sid): 135303826
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Other Information:
FDA Substances Added to Food (formerly EAFUS): View
HMDB (The Human Metabolome Database): Search
FooDB: FDB008946
FDA Listing of Food Additive Status: View
VCF-Online: VCF Volatile Compounds in Food
Wikipedia: View
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Potential Blenders and core components note
 
None Found
 
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Potential Uses:
None Found
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Occurrence (nature, food, other): note
 amylase
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 bacillus stearothermophilus
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Synonyms:
alpha-amylase enzyme preparation from bacillus stearothermophilus
 bacillus stearothermophilus alpha-amylase
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Articles:
PubMed: Three-dimensional structure of a variant `Termamyl-like' Geobacillus stearothermophilus α-amylase at 1.9 Å resolution.
PubMed: Effect of codon-optimized E. coli signal peptides on recombinant Bacillus stearothermophilus maltogenic amylase periplasmic localization, yield and activity.
PubMed: Combined impact of Bacillus stearothermophilus maltogenic alpha-amylase and surfactants on starch pasting and gelation properties.
PubMed: Expression and Characterization of Geobacillus stearothermophilus SR74 Recombinant α-Amylase in Pichia pastoris.
PubMed: Novel thermostable and alkalitolerant amylase production by Geobacillus stearothermophilus HP 3.
PubMed: Sequence and structural investigation of a novel psychrophilic α-amylase from Glaciozyma antarctica PI12 for cold-adaptation analysis.
PubMed: Characterisation of three starch degrading enzymes: thermostable β-amylase, maltotetraogenic and maltogenic α-amylases.
PubMed: Enzymatic synthesis of glycosylated puerarin using maltogenic amylase from Bacillus stearothermophilus expressed in Bacillus subtilis.
PubMed: Impact of amylases on biopolymer dynamics during storage of straight-dough wheat bread.
PubMed: Structural and biochemical features of acidic α-amylase of Bacillus acidicola.
PubMed: Phylogenetic and Comparative Sequence Analysis of Thermostable Alpha Amylases of kingdom Archea, Prokaryotes and Eukaryotes.
PubMed: The S-layer from Bacillus stearothermophilus DSM 2358 functions as an adhesion site for a high-molecular-weight amylase.
PubMed: Bacillus stearothermophilus contains a plasmid-borne gene for alpha-amylase.
PubMed: Structural genes encoding the thermophilic alpha-amylases of Bacillus stearothermophilus and Bacillus licheniformis.
PubMed: C-terminal truncations of a thermostable Bacillus stearothermophilus alpha-amylase.
PubMed: Expression of the insecticidal protein gene from Bacillus thuringiensis subsp. aizawai in Bacillus subtilis and in the thermophile Bacillus stearothermophilus by using the alpha-amylase promoter of the thermophile.
PubMed: Efficient synthesis and secretion of a thermophilic alpha-amylase by protein-producing Bacillus brevis 47 carrying the Bacillus stearothermophilus amylase gene.
PubMed: Evidence that an N-terminal S-layer protein fragment triggers the release of a cell-associated high-molecular-weight amylase in Bacillus stearothermophilus ATCC 12980.
PubMed: Toward the smallest active subdomain of a TIM-barrel fold: insights from a truncated α-amylase.
PubMed: Safety evaluation of highly-branched cyclic dextrin and a 1,4-alpha-glucan branching enzyme from Bacillus stearothermophilus.
PubMed: Cloning and expression of an amylase gene from Bacillus stearothermophilus.
PubMed: The production of alpha-amylase in batch and chemostat culture by Bacillus stearothermophilus.
PubMed: Cloning and expression of a thermophilic alpha-amylase gene from Bacillus stearothermophilus in Escherichia coli.
PubMed: Efficient production of Bacillus stearothermophilus alpha-amylase in Bacillus brevis by altering its signal peptide.
PubMed: Evidence for movement of the alpha-amylase gene into two phylogenetically distant Bacillus stearothermophilus strains.
PubMed: Thermostability enhancement and change in starch hydrolysis profile of the maltohexaose-forming amylase of Bacillus stearothermophilus US100 strain.
PubMed: Characterization of a thermostable Bacillus stearothermophilus alpha-amylase.
PubMed: The high-molecular-mass amylase (HMMA) of Geobacillus stearothermophilus ATCC 12980 interacts with the cell wall components by virtue of three specific binding regions.
PubMed: Site-directed mutagenesis of a thermostable alpha-amylase from Bacillus stearothermophilus: putative role of three conserved residues.
PubMed: Effects of signal peptide mutations on processing of Bacillus stearothermophilus alpha-amylase in Escherichia coli.
PubMed: Cloning of a chromosomal alpha-amylase gene from Bacillus stearothermophilus.
PubMed: SAMY, a novel mammalian reporter gene derived from Bacillus stearothermophilus alpha-amylase.
PubMed: Alpha-factor-directed synthesis of Bacillus stearothermophilus alpha-amylase in Saccharomyces cerevisiae.
PubMed: Subchronic toxicity studies in dogs and in utero rats fed diets containing Bacillus stearothermophilus alpha-amylase from a natural or recombinant DNA host.
PubMed: Nucleotide sequence of the Bacillus stearothermophilus alpha-amylase gene.
PubMed: Multiple amylase genes in two strains of Bacillus stearothermophilus.
PubMed: Fusion of Bacillus stearothermophilus leucine aminopeptidase II with the raw-starch-binding domain of Bacillus sp. strain TS-23 alpha-amylase generates a chimeric enzyme with enhanced thermostability and catalytic activity.
PubMed: Purification and characterization of a thermostable alpha-amylase from Bacillus stearothermophilus.
PubMed: Cloning and expression of the alpha-amylase gene from Bacillus stearothermophilus in several staphylococcal species.
PubMed: Single-stranded DNA plasmid, vector construction and cloning of Bacillus stearothermophilus alpha-amylase in Lactobacillus.
PubMed: Engineering of the alpha-amylase from Geobacillus stearothermophilus US100 for detergent incorporation.
PubMed: Co-overexpression of prlF increases cell viability and enzyme yields in recombinant Escherichia coli expressing Bacillus stearothermophilus alpha-amylase.
PubMed: Transglycosylation reactions of Bacillus stearothermophilus maltogenic amylase with acarbose and various acceptors.
PubMed: Preliminary X-ray crystallographic analysis of a novel maltogenic amylase from Bacillus stearothermophilus ET1.
PubMed: X-ray structure of Novamyl, the five-domain "maltogenic" alpha-amylase from Bacillus stearothermophilus: maltose and acarbose complexes at 1.7A resolution.
PubMed: Random mutagenesis used to probe the structure and function of Bacillus stearothermophilus alpha-amylase.
PubMed: Effect of mutation of an amino acid residue near the catalytic site on the activity of Bacillus stearothermophilus alpha-amylase.
PubMed: Crystal structure of Bacillus stearothermophilus alpha-amylase: possible factors determining the thermostability.
PubMed: [Isolation, purification and characteristics of alpha-amylase from Bacillus stearothermophilus].
PubMed: Investigation of the mechanisms of irreversible thermoinactivation of Bacillus stearothermophilus alpha-amylase.
PubMed: [Extracellular thermostable alpha-amylase from Bacillus stearothermophilus Q8].
PubMed: Purification, crystallization and preliminary X-ray crystallographic study of alpha-amylase from Bacillus stearothermophilus.
PubMed: The importance of an extra loop in the B-domain of an alpha-amylase from B. stearothermophilus US100.
PubMed: Role of calcium ion in the thermostability of alpha-amylase produced from Bacillus stearothermophilus.
PubMed: Random point mutation analysis of the signal peptide cleavage area of Bacillus stearothermophilus alpha-amylase.
PubMed: Maltogenic amylases of Bacillus stearothermophilus.
PubMed: Structure and expression of an amylopullulanase gene from Bacillus stearothermophilus TS-23.
PubMed: Properties and active center of the thermostable branching enzyme from Bacillus stearothermophilus.
PubMed: Nucleotide sequence of the neopullulanase gene from Bacillus stearothermophilus.
PubMed: Maltosyl-erythritol, a major transglycosylation product of erythritol by Bacillus stearothermophilus maltogenic amylase.
PubMed: Modification of ascorbic acid using transglycosylation activity of Bacillus stearothermophilus maltogenic amylase to enhance its oxidative stability.
PubMed: Transglycosylation of neohesperidin dihydrochalcone by Bacillus stearothermophilus maltogenic amylase.
PubMed: Conjugal transfer of recombinant transposon Tn916 from Escherichia coli to Bacillus stearothermophilus.
PubMed: Bacillus stearothermophilus neopullulanase selective hydrolysis of amylose to maltose in the presence of amylopectin.
PubMed: Temperature-sensitive binding of alpha-glucans by Bacillus stearothermophilus.
PubMed: Transglycosylation of glycosyl residues to cyclic tetrasaccharide by Bacillus stearothermophilus cyclomaltodextrin glucanotransferase using cyclomaltodextrin as the glycosyl donor.
PubMed: Analysis of the active center of Bacillus stearothermophilus neopullulanase.
PubMed: Analysis of the structure-function relationship of the S-layer protein SbsC of Bacillus stearothermophilus ATCC 12980 by producing truncated forms.
PubMed: Reddish Escherichia coli cells caused by overproduction of Bacillus stearothermophilus uroporphyrinogen III methylase: cloning, sequencing, and expression of the gene.
PubMed: Bacillus stearothermophilus from Saudi Arabian Soils.
PubMed: Three-dimensional structure and substrate binding of Bacillus stearothermophilus neopullulanase.
PubMed: Production and characterization of thermostable alpha-amylase by thermophilic Geobacillus stearothermophilus.
PubMed: Structure of the cyclic glucan produced from amylopectin by Bacillus stearothermophilus branching enzyme.
PubMed: Enhanced secretion of Bacillus stearothermophilus L1 lipase in Saccharomyces cerevisiae by translational fusion to cellulose-binding domain.
PubMed: EFFECT OF CARBON SOURCES ON FORMATION OF ALPHA-AMYLASE BY BACILLUS STEAROTHERMOPHILUS.
PubMed: PREFERENTIAL SYNTHESIS OF ALPHA-AMYLASE BY BACILLUS STEAROTHERMOPHILUS IN THE PRESENCE OF 5-METHYL-TRYPTOPHAN.
PubMed: Thermostable glucokinase from Bacillus stearothermophilus and its analytical application.
PubMed: Construction of a vector plasmid that can be maintained stably at higher temperatures in Bacillus stearothermophilus and its application.
PubMed: Coproduction of thermostable amylase and beta-galactosidase enzymes by Geobacillus stearothermophilus SAB-40: aplication of Plackett-Burman design to evaluate culture requirements affecting enzyme production.
PubMed: INDUCTION OF ALPHA-AMYLASE OF BACILLUS STEAROTHERMOPHILUS BY MALTODEXTRINS.
PubMed: DE NOVO SYNTHESIS OF ALPHA-AMYLASE BY BACILLUS STEAROTHERMOPHILUS.
PubMed: Microencapsulation of maltogenic α-amylase in poly(urethane-urea) shell: inverse emulsion method.
PubMed: INDUCED BIOSYNTHESIS OF ALPHA-AMYLASE BY GROWING CULTURES OF BACILLUS STEAROTHERMOPHILUS.
PubMed: Protein enrichment of corn cob heteroxylan waste slurry by thermophilic aerobic digestion using Bacillus stearothermophilus.
PubMed: Complete nucleotide sequence of a thermophilic alpha-amylase gene: homology between prokaryotic and eukaryotic alpha-amylases at the active sites.
PubMed: Molecular and enzymatic characterization of a maltogenic amylase that hydrolyzes and transglycosylates acarbose.
PubMed: Cloning and Expression of Thermostable alpha-Amylase Gene from Bacillus stearothermophilus in Bacillus stearothermophilus and Bacillus subtilis.
PubMed: Thermostable alpha-amylase of Bacillus stearothermophilus. II. Physical properties and molecular weight.
PubMed: Thermostable alpha-amylase of Bacillus stearothermophilus. III. Amino acid composition.
PubMed: Mechanisms of irreversible thermal inactivation of Bacillus alpha-amylases.
PubMed: Why is one Bacillus alpha-amylase more resistant against irreversible thermoinactivation than another?
PubMed: Studies on thermophilic alpha-amylase from Bacillus stearothermophilus. I. Some general and physico-chemical properties of thermophilic alpha-amylase.
PubMed: Studies on thermophilic alpha-amylase from Bacillus stearothermophilus. 3. Effect of temperature on the renaturation of denatured thermophilic alpha-amylase.
PubMed: The extracellular alpha-amylase of bacillus stearothermophilus.
PubMed: Studies on thermophilic alpha-amylase from Bacillus stearothermophilus. II. Thermal stability of thermophilic alpha-amylase.
PubMed: Enzymological differences of alpha-amylase from Bacillus stearothermophilus grown at 37 degrees C and 55 degrees C.
PubMed: Role of Val289 residue in the alpha-amylase of Bacillus amyloliquefaciens MTCC 610: an analysis by site directed mutagenesis.
PubMed: Thermostable alpha-amylase of Bacillus stearothermophilus. IV. Amino-terminal and carboxyl-terminal amino acid analysis.
PubMed: Biochemical changes in lysogenic Bacillus stearothermophilus after bacteriophage induction.
PubMed: Temperature impacts the multiple attack action of amylases.
PubMed: Immunochemical studies on alpha-amylase. 3. Immunochemical relationships among amylases from various microorganisms.
PubMed: Culture Conditions for Production of Thermostable Amylase by Bacillus stearothermophilus.
PubMed: Production of alpha-amylase in fed-batch cultures of vgb+ and vgb- recombinant Escherichia coli: some observations.
PubMed: Efficacy of supplementation of alpha-amylase-producing bacterial culture on the performance, nutrient use, and gut morphology of broiler chickens fed a corn-based diet.
PubMed: Alcoholysis reactions from starch with alpha-amylases.
PubMed: Complete nucleotide sequence of a gene coding for heat- and pH-stable alpha-amylase of Bacillus licheniformis: comparison of the amino acid sequences of three bacterial liquefying alpha-amylases deduced from the DNA sequences.
PubMed: Presence of the bacterial hemoglobin gene improves alpha-amylase production of a recombinant Escherichia coli strain.
PubMed: Expression of a bacterial alpha-amylase gene in transgenic rice seeds.
PubMed: Thermostable alpha-amylase of Bacillus stearothermophilus. I. Crystallization and some general properties.
PubMed: Bacillus stearothermophilus. II. Certain factors affecting amylase production on some undefined media.
PubMed: Bacillus stearothermophilus. I. Thermal and pH stability of the amylase.
PubMed: Thermostable, Raw-Starch-Digesting Amylase from Bacillus stearothermophilus.
PubMed: Evaluation of column flotation in the downstream processing of fermentation products: recovery of a genetically engineered alpha-amylase.
PubMed: Extracellular production of cloned alpha-amylase by Escherichia coli.
PubMed: Cloning of the maltose phosphorylase gene from Bacillus sp. strain RK-1 and efficient production of the cloned gene and the trehalose phosphorylase gene from Bacillus stearothermophilus SK-1 in Bacillus subtilis.
PubMed: An enzymatically produced novel cyclomaltopentaose cyclized from amylose by an alpha-(1-->6)-linkage, cyclo-{-->6)-alpha-D-Glcp-(1-->4)-alpha-D-Glcp-(1-->4)-alpha-D-Glcp-(1-->4)-alpha-D-Glcp-(1-->4)-alpha-D-Glcp-(1-->}.
PubMed: Introducing transglycosylation activity in a liquefying alpha-amylase.
PubMed: Engineering cyclodextrin glycosyltransferase into a starch hydrolase with a high exo-specificity.
PubMed: Transglycosylation of naringin by Bacillus stearothermophilusMaltogenic amylase to give glycosylated naringin.
PubMed: Enzymatic synthesis of a new inhibitor of alpha-amylases: acarviosinyl-isomaltosyl-spiro-thiohydantoin.
PubMed: Functional analysis of the Lactococcus lactis usp45 secretion signal in the secretion of a homologous proteinase and a heterologous alpha-amylase.
PubMed: An enzymatic method for the alpha-amylase assay which comprises a new procedure for eliminating glucose and maltose in biological fluids.
PubMed: Use of a new catabolite repression resistant promoter isolated from Bacillus subtilis KCC103 for hyper-production of recombinant enzymes.
PubMed: Production of alpha-amylase in transformant of bacillus stearothermophilus-improvement of recombinant plasmid that can be used at higher temperatures.
PubMed: Secretion of heterologous proteins in Bacillus subtilis can be improved by engineering cell components affecting post-translocational protein folding and degradation.
PubMed: Engineering direct fructose production in processed potato tubers by expressing a bifunctional alpha-amylase/glucose isomerase gene complex.
PubMed: Effect of glycosylation of bacterial amylase on stability and active site conformation.
PubMed: Study of stability of recombinant plasmids during the continuous culture of Bacillus stearothermophilus NUB3621 in nonselective medium.
PubMed: Effects of moranoline, 4-O-alpha-D-glucopyranosylmoranoline and their N-substituted derivatives on thermostability of cyclodextrin glycosyltransferase, glucoamylase, and beta-amylase.
PubMed: Enzymatic properties of a novel liquefying alpha-amylase from an alkaliphilic Bacillus isolate and entire nucleotide and amino acid sequences.
PubMed: Differential heat stabilities of Bacillus amylase.
PubMed: An Escherichia coli host strain useful for efficient overproduction of secreted recombinant protein.
PubMed: Secretion of Bacillus alpha-amylase from yeast directed by glucoamylase I signal sequence of Saccharomyces diastaticus.
PubMed: Deduced amino-acid sequence of a calcium-free alpha-amylase from a strain of Bacillus: implications from molecular modeling of high oxidation stability and chelator resistance of the enzyme.
PubMed: Conserved residues of liquefying alpha-amylases are concentrated in the vicinity of active site.
PubMed: Integrative vector for constructing single-copy translational fusions between regulatory regions of Bacillus subtilis and the bgaB reporter gene encoding a heat-stable beta-galactosidase.
PubMed: Structural consequences of neopullulanase mutations.
PubMed: Hydrolysis of amylopectin by amylolytic enzymes: level of inner chain attack as an important analytical differentiation criterion.
PubMed: In vitro enzymatic modification of puerarin to puerarin glycosides by maltogenic amylase.
PubMed: Inhibition of beta-glycosidases by acarbose analogues containing cellobiose and lactose structures.
PubMed: Hydrolysis of amylopectin by amylolytic enzymes: structural analysis of the residual amylopectin population.
PubMed: Molecular weight of thermostable alpha-amylase from B. stearothermophilus.
PubMed: Protein processing to form extracellular thermostable alpha-amylases from a gene fused in a Bacillus subtilis secretion vector.
PubMed: Comparison of thermostable alpha-amylases from B. stearothermophilus grown at different temperatures.
PubMed: Heterologous expression, secretion and characterization of the Geobacillus thermoleovorans US105 type I pullulanase.
PubMed: Does the increased hydrophobicity of the interior and hydrophilicity of the exterior of an enzyme structure reflect its increased thermostability?
PubMed: [Thermostability of alpha-amylase produced from thermophilic bacterium (author's transl)].
PubMed: Identification of new secreted proteins and secretion of heterologous amylase by C. glutamicum.
PubMed: Heterologous protein production capacity of mammalian cells cultivated as monolayers and microtissues.
PubMed: [Study on excess sludge solubilization in S-TE process under microaeration].
PubMed: Catalytic activities of intracellular dimeric neopullulanase on cyclodextrin, acarbose and maltose.
PubMed: [Transglycosylation of benzo[h]quinazolines].
PubMed: Artificial insertion of peptides between signal peptide and mature protein: effect on secretion and processing of hybrid thermostable alpha-amylases in Bacillus subtilis and Escherichia coli cells.
PubMed: Enzymatic synthesis and properties of highly branched rice starch amylose and amylopectin cluster.
PubMed: Crystal structure of a maltogenic amylase provides insights into a catalytic versatility.
PubMed: Cyclization reaction catalyzed by branching enzyme.
PubMed: Synthesis of glycosyl-trehaloses by cyclomaltodextrin glucanotransferase through the transglycosylation reaction.
PubMed: Comparative study of the inhibition of alpha-glucosidase, alpha-amylase, and cyclomaltodextrin glucanosyltransferase by acarbose, isoacarbose, and acarviosine-glucose.
PubMed: Role of the glutamate 332 residue in the transglycosylation activity of ThermusMaltogenic amylase.
PubMed: Nucleotide sequence of the alpha-amylase-pullulanase gene from Clostridium thermohydrosulfuricum.
PubMed: Solubilization of organic sludge by thermophilic aerobic bacteria as a pretreatment for anaerobic digestion.
PubMed: pBRINT-Ts: a plasmid family with a temperature-sensitive replicon, designed for chromosomal integration into the lacZ gene of Escherichia coli.
PubMed: Antifirming effects of starch degrading enzymes in bread crumb.
PubMed: Integration and expression of alpha-amylase and endoglucanase genes in the Lactobacillus plantarum chromosome.
PubMed: Comparison of primary structures and substrate specificities of two pullulan-hydrolyzing alpha-amylases, TVA I and TVA II, from Thermoactinomyces vulgaris R-47.
PubMed: Development of an arming yeast strain for efficient utilization of starch by co-display of sequential amylolytic enzymes on the cell surface.
PubMed: Simultaneous comparison of several sequences.
PubMed: Scanning the Corynebacterium glutamicum R genome for high-efficiency secretion signal sequences.
PubMed: Construction of high, intermediate and low-copy-number promoter-probe plasmids for Bacillus subtilis.
PubMed: [Thermophilic bacteria from hot springs of Kamchatka].
PubMed: A novel alpha-glucosidase inhibitor from pine bark.
PubMed: Cooperative action of alpha-glucanotransferase and maltogenic amylase for an improved process of isomaltooligosaccharide (IMO) production.
PubMed: Evaluation of magnetic resonance for detection of bacterial contamination in low-acid, shelf-stable packaged soymilk.
PubMed: Mechanisms of thermophily.
PubMed: Antioxidative effects of glycosyl-ascorbic acids synthesized by maltogenic amylase to reduce lipid oxidation and volatiles production in cooked chicken meat.
PubMed: Some factors affecting the copy number of specific plasmids in Bacillus species.
PubMed: In vivo genetic engineering: homologous recombination as a tool for plasmid construction.
PubMed: Effect of temperature on the saccharide composition obtained after alpha-amylolysis of starch
PubMed: Nucleotide sequence of the cryptic plasmid pTT8 from Thermus thermophilus HB8 and isolation and characterization of its high-copy-number mutant.
PubMed: Characterization of three putative Lon proteases of Thermus thermophilus HB27 and use of their defective mutants as hosts for production of heterologous proteins.
PubMed: Synthesis of glycosyl glycerol by cyclodextrin glucanotransferases.
PubMed: InXy and SeXy, compact heterologous reporter proteins for mammalian cells.
PubMed: Cell surface engineering of yeast: construction of arming yeast with biocatalyst.
PubMed: Construction of an Escherichia coli export-affinity vector for expression and purification of foreign proteins by fusion to cyclomaltodextrin glucanotransferase.
PubMed: Isolation of alpha-glucosidase-producing thermophilic bacilli from hot springs of Turkey.
PubMed: A novel synthetic mammalian promoter derived from an internal ribosome entry site.
PubMed: Autoregulated, bidirectional and multicistronic gas-inducible mammalian as well as lentiviral expression vectors.
PubMed: New-generation multicistronic expression platform: pTRIDENT vectors containing size-optimized IRES elements enable homing endonuclease-based cistron swapping into lentiviral expression vectors.
PubMed: Functions of S-layers.
PubMed: Adenoviral vector platform for transduction of constitutive and regulated tricistronic or triple-transcript transgene expression in mammalian cells and microtissues.
PubMed: Genetic immobilization of proteins on the yeast cell surface.
PubMed: A nine-residue synthetic propeptide enhances secretion efficiency of heterologous proteins in Lactococcus lactis.
PubMed: Separation and characterization of five positional isomers of trimaltosyl-cyclomaltoheptaose (trimaltosyl-beta-cyclodextrin).
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