alpha-amylase (thermostable) from bacillus licheniformis
 
Notes:
enzymes that catalyze the endohydrolysis of 1,4-alpha-glycosidic linkages in starch; glycogen; and related polysaccharides and oligosaccharides containing 3 or more 1,4-alpha-linked d-glucose units.
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CAS Number: 9000-85-5 
ECHA EC Number: 232-560-9
MDL: MFCD00081319
Formula: unspecified
Category: starch hydrolysis
 
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JECFA Food Additive: alpha-Amylase (thermostable) from Bacillus licheniformis
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Physical Properties:
Appearance: brown liquid (est)
Food Chemicals Codex Listed: No
Storage: refrigerate in tightly sealed containers.
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Organoleptic Properties:
 
Odor and/or flavor descriptions from others (if found).
 
 
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Cosmetic Information:
None found
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Suppliers:
Sigma-Aldrich: Sigma
For experimental / research use only.
a-Amylase from Bacillus licheniformis lyophilized powder, 500-1,500 units/mg protein, 93-100% (SDS-PAGE)
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Safety Information:
Preferred SDS: View
European information :
Most important hazard(s):
Xn - Harmful.
R 42 - May cause sensitisation by inhalation.
S 02 - Keep out of the reach of children.
S 37/39 - Wear suitable gloves and eye/face protection.
 
Hazards identification
 
Classification of the substance or mixture
GHS Classification in accordance with 29 CFR 1910 (OSHA HCS)
None found.
GHS Label elements, including precautionary statements
 
Pictogram
 
Hazard statement(s)
None found.
Precautionary statement(s)
None found.
Oral/Parenteral Toxicity:
Not determined
Dermal Toxicity:
Not determined
Inhalation Toxicity:
Not determined
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Safety in Use Information:
Category: starch hydrolysis
Recommendation for alpha-amylase (thermostable) from bacillus licheniformis usage levels up to:
 not for fragrance use.
 
Recommendation for alpha-amylase (thermostable) from bacillus licheniformis flavor usage levels up to:
 not for flavor use.
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Safety References:
European Food Safety Authority (EFSA) reference(s):
Explanatory Note for the Guidance of the Scientific Panel of Food Contact Material, Enzymes, Flavourings and Processing Aids (CEF) on the Submission of a Dossier on Food Enzymes
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Scientific Opinion on the efficacy of Ronozyme® Rumistar (alpha-amylase) as a feed additive for dairy cows
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Safety evaluation of the food enzyme a-amylase from Bacillus licheniformis (strain DP-Dzb44)
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Safety evaluation of the food enzyme a-amylase from a genetically modified strain of Bacillus licheniformis (DP-Dzb25)
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EPA Substance Registry Services (TSCA): 9000-85-5
EPA ACToR: Toxicology Data
EPA Substance Registry Services (SRS): Registry
National Institute of Allergy and Infectious Diseases: Data
WGK Germany: 3
Chemidplus: 0009000855
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References:
Pubchem (sid): 135337197
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Other Information:
HMDB (The Human Metabolome Database): Search
FDA Listing of Food Additive Status: View
VCF-Online: VCF Volatile Compounds in Food
Wikipedia: View
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Potential Blenders and core components note
 
None Found
 
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Potential Uses:
None Found
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Occurrence (nature, food, other): note
 amylase
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 bacillus licheniformis
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Synonyms:
alpha-amylase (thermostable) from bacillus licheniformis
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Articles:
PubMed: Introducing transglycosylation activity in Bacillus licheniformis α-amylase by replacement of His235 with Glu.
J-Stage: Does Recovery in the Spectral Characteristics of GdnHCl-Denatured Bacillus licheniformis a-Amylase Due to Added Calcium Point towards Protein Stabilization?
PubMed: Engineering a repression-free catabolite-enhanced expression system for a thermophilic alpha-amylase from Bacillus licheniformis MSG.
PubMed: Experimental evidence for a 9-binding subsite of Bacillus licheniformis thermostable α-amylase.
PubMed: A highly active alpha amylase from Bacillus licheniformis: directed evolution, enzyme characterization and structural analysis.
PubMed: Efficient expression of nattokinase in Bacillus licheniformis: host strain construction and signal peptide optimization.
PubMed: Escherichia coli signal peptidase recognizes and cleaves the signal sequence of α-amylase originating from Bacillus licheniformis.
PubMed: Molten globule-like partially folded state of Bacillus licheniformis α-amylase at low pH induced by 1,1,1,3,3,3-hexafluoroisopropanol.
PubMed: Overcoming hydrolysis of raw corn starch under industrial conditions with Bacillus licheniformis ATCC 9945a α-amylase.
PubMed: Nanoactivator mediated modifications in thermostable amylase from Bacillus licheniformis.
PubMed: Analysis of a preferential action of α-amylase from B. licheniformis towards amorphous regions of waxy maize starch.
PubMed: New insights into the effectiveness of alpha-amylase enzyme presentation on the Bacillus subtilis spore surface by adsorption and covalent immobilization.
PubMed: N-Glycosylation deficiency enhanced heterologous production of a Bacillus licheniformis thermostable α-amylase in Saccharomyces cerevisiae.
PubMed: Codon Optimization Significantly Improves the Expression Level of α -Amylase Gene from Bacillus licheniformis in Pichia pastoris.
PubMed: Characterization of the native form and the carboxy-terminally truncated halotolerant form of α-amylases from Bacillus subtilis strain FP-133.
PubMed: Does recovery in the spectral characteristics of GdnHCl-denatured Bacillus licheniformis α-amylase due to added calcium point towards protein stabilization?
PubMed: A proteomic view of cell physiology of the industrial workhorse Bacillus licheniformis.
PubMed: Improvement of the acid stability of Bacillus licheniformis alpha amylase by error-prone PCR.
PubMed: Enzymatic characterization of Bacillus licheniformis γ-glutamyltranspeptidase fused with N-terminally truncated forms of Bacillus sp. TS-23 α-amylase.
PubMed: Bacillus licheniformis BT5.9 Isolated from Changar Hot Spring, Malang, Indonesia, as a Potential Producer of Thermostable α-amylase.
PubMed: Significance of Tyr302, His235 and Asp194 in the α-amylase from Bacillus licheniformis.
PubMed: Solid State Fermentation of a Raw Starch Digesting Alkaline Alpha-Amylase from Bacillus licheniformis RT7PE1 and Its Characteristics.
PubMed: Mathematical modeling of maize starch liquefaction catalyzed by α-amylases from Bacillus licheniformis: effect of calcium, pH and temperature.
PubMed: Isolation and characterization of different strains of Bacillus licheniformis for the production of commercially significant enzymes.
PubMed: Structural properties of hydrolyzed high-amylose rice starch by α-amylase from Bacillus licheniformis.
PubMed: Developing a new production host from a blueprint: Bacillus pumilus as an industrial enzyme producer.
PubMed: Role of two amino acid residues' insertion on thermal stability of thermophilic α-amylase AMY121 from a deep sea bacterium Bacillus sp. SCSIO 15121.
PubMed: Role of the salt bridge between Arg176 and Glu126 in the thermal stability of the Bacillus amyloliquefaciens α-amylase (BAA).
PubMed: Investigation on the effects of three X-->histidine replacements on thermostability of alpha-amylase from Bacillus amyloliquefaciens.
PubMed: Comparative study on production of a-Amylase from Bacillus licheniformis strains.
PubMed: An analysis of temperature adaptation in cold active, mesophilic and thermophilic Bacillus α-amylases.
PubMed: Repeated Random Mutagenesis of alpha-Amylase from Bacillus licheniformis for Improved pH Performance.
PubMed: Phylogenetic and Comparative Sequence Analysis of Thermostable Alpha Amylases of kingdom Archea, Prokaryotes and Eukaryotes.
PubMed: Effects of alpha-amylase reaction mechanisms on analysis of resistant-starch contents.
PubMed: Immobilization of Bacillus licheniformis α-amylase onto reactive polymer films.
PubMed: An investigation into the preservation of microbial cell banks for α-amylase production during 5 l fed-batch Bacillus licheniformis fermentations.
PubMed: Chicken feathers: a complex substrate for the co-production of alpha-amylase and proteases by B. licheniformis NH1.
PubMed: Cloning and extracellular expression of a raw starch digesting α-amylase (Blamy-I) and its application in bioethanol production from a non-conventional source of starch.
PubMed: Conformational destabilization of Bacillus licheniformis α-amylase induced by lysine modification and calcium depletion.
PubMed: Effective solubilization and single-step purification of Bacillus licheniformis alpha-amylase from insoluble aggregates.
PubMed: Mixed-mode resins: taking shortcut in downstream processing of raw-starch digesting α-amylases.
PubMed: A novel alpha-amylase from Bacillus mojavensis A21: purification and biochemical characterization.
PubMed: Characterisation and identification of enzyme-producing autochthonous bacteria from the gastrointestinal tract of two Indian air-breathing fish.
PubMed: High yield recombinant thermostable alpha-amylase production using an improved Bacillus licheniformis system.
PubMed: Contribution of protein, starch, and fat to the apparent ileal digestible energy of corn- and wheat-based broiler diets in response to exogenous xylanase and amylase without or with protease.
PubMed: In vivo self-assembly of stable green fluorescent protein fusion particles and their uses in enzyme immobilization.
PubMed: Complete nucleotide sequence of a gene coding for heat- and pH-stable alpha-amylase of Bacillus licheniformis: comparison of the amino acid sequences of three bacterial liquefying alpha-amylases deduced from the DNA sequences.
PubMed: Genetic improvement of alpha-amylase producing Bacillus licheniformis by homolog-mediated alpha-amylase gene amplification.
PubMed: Enzymatic synthesis of glycosylated puerarin using maltogenic amylase from Bacillus stearothermophilus expressed in Bacillus subtilis.
PubMed: Role of Val289 residue in the alpha-amylase of Bacillus amyloliquefaciens MTCC 610: an analysis by site directed mutagenesis.
PubMed: Phylogenetic and biochemical characterization of a new halo-thermotolerant, biofilm-forming Bacillus from Saline Lake of Iran.
PubMed: Functional analysis of the response regulator DegU in Bacillus megaterium DSM319 and comparative secretome analysis of degSU mutants.
PubMed: Comparison of starch hydrolysis activity and thermal stability of two Bacillus licheniformis alpha-amylases and insights into engineering alpha-amylase variants active under acidic conditions.
PubMed: 2,3-butanediol production from starch by engineered Klebsiella pneumoniae G31-A.
PubMed: Co-production of surfactin and a novel bacteriocin by Bacillus subtilis subsp. subtilis H4 isolated from Bikalga, an African alkaline Hibiscus sabdariffa seed fermented condiment.
PubMed: Crystal structure of thermostable alpha-amylase from Bacillus licheniformis refined at 1.7 A resolution.
PubMed: N-terminal amino acid sequence of Bacillus licheniformis alpha-amylase: comparison with Bacillus amyloliquefaciens and Bacillus subtilis Enzymes.
PubMed: [Cloning of the gene encoding a thermostable alpha-amylase from bacillus licheniformis CICIM B0204 and functional identification of its promoter].
PubMed: Regulatory factors affecting alpha-amylase production in bacillus licheniformis.
PubMed: Effect of oilseed cakes on alpha-amylase production by Bacillus licheniformis CUMC305.
PubMed: Structure of Bacillus amyloliquefaciens alpha-amylase at high resolution: implications for thermal stability.
PubMed: Site-directed mutagenesis of the calcium-binding site of alpha-amylase of Bacillus licheniformis.
PubMed: Functional half-life of the alpha-amylase mRNA of Bacillus licheniformis.
PubMed: Hybrid Bacillus amyloliquefaciens X Bacillus licheniformis alpha-amylases. Construction, properties and sequence determinants.
PubMed: Synthesis of alpha-amylase and alpha-glucosidase by membrane bound ribosomes from Bacillus licheniformis.
PubMed: A natural variant of Bacillus licheniformis alpha-amylase isolated from flour mill wastewaters sheds light on the origin of high thermostability.
PubMed: Alpha-amylase from Bacillus licheniformis mutants near to the catalytic site: effects on hydrolytic and transglycosylation activity.
PubMed: Catalytic properties of the cloned amylase from Bacillus licheniformis.
PubMed: Structural genes encoding the thermophilic alpha-amylases of Bacillus stearothermophilus and Bacillus licheniformis.
PubMed: Molecular cloning in Bacillus subtilis of a Bacillus licheniformis gene encoding a thermostable alpha amylase.
PubMed: Use of amber suppressors to investigate the thermostability of Bacillus licheniformis alpha-amylase. Amino acid replacements at 6 histidine residues reveal a critical position at His-133.
PubMed: Isolation and the 5'-end nucleotide sequence of Bacillus licheniformis alpha-amylase gene.
PubMed: Acid stabilization of Bacillus licheniformis alpha amylase through introduction of mutations.
PubMed: High expression of Bacillus licheniformis alpha-amylase with a Bacillus secretion vector.
PubMed: New strains of Bacillus licheniformis and Bacillus coagulans producing thermostable alpha-amylase active at alkaline pH.
PubMed: Probing structural determinants specifying high thermostability in Bacillus licheniformis alpha-amylase.
PubMed: Expression of alpha-amylase in Bacillus licheniformis.
PubMed: Crystal structure of calcium-depleted Bacillus licheniformis alpha-amylase at 2.2 A resolution.
PubMed: Correlation between alpha-amylase production and sporulation in Bacillus licheniformis CUMC 305 with respect to the effect of some carbohydrates and phenylmethylsulfonyl fluoride treatment.
PubMed: Bacillus licheniformis alpha-amylase gene, amyL, is subject to promoter-independent catabolite repression in Bacillus subtilis.
PubMed: Activation of Bacillus licheniformis alpha-amylase through a disorder-->order transition of the substrate-binding site mediated by a calcium-sodium-calcium metal triad.
PubMed: Thermostable alpha-amylase production using Bacillus licheniformis NRRL B14368.
PubMed: Chemical modification of lysine residues in Bacillus licheniformis alpha-amylase: conversion of an endo- to an exo-type enzyme.
PubMed: Production of active Bacillus licheniformis alpha-amylase in tobacco and its application in starch liquefaction.
PubMed: Nucleotide sequence of the 5' region of the Bacillus licheniformis alpha-amylase gene: comparison with the B. amyloliquefaciens gene.
PubMed: Hyperthermostable mutants of Bacillus licheniformis alpha-amylase: multiple amino acid replacements and molecular modelling.
PubMed: Synthesis and processing of Escherichia coli TEM-beta-lactamase and Bacillus licheniformis alpha-amylase in E. coli: the role of signal peptidase I.
PubMed: Pearl millet, a source of alpha amylase production by Bacillus licheniformis.
PubMed: Effect of temperature on subsite map of Bacillus licheniformis alpha-amylase.
PubMed: Kinetic study of the irreversible thermal denaturation of Bacillus licheniformis alpha-amylase.
PubMed: Production of alpha amylase by Bacillus licheniformis using an economical medium.
PubMed: [Extracellular thermostable alpha-amylase from Bacillus licheniformis B7].
PubMed: Hyperthermostabilization of Bacillus licheniformis alpha-amylase and modulation of its stability over a 50 degrees C temperature range.
PubMed: Expression and secretion of an alpha-amylase gene from a native strain of Bacillus licheniformis in Escherichia coli by T7 promoter and putative signal peptide of the gene.
PubMed: A thermophilic extracellular -amylase from Bacillus licheniformis.
PubMed: [Preparation of an active strain of Bacillus licheniformis--producer of thermostable alpha-amylase].
PubMed: Action pattern and subsite mapping of Bacillus licheniformis alpha-amylase (BLA) with modified maltooligosaccharide substrates.
PubMed: Convenience of immobilized Bacillus licheniformis alpha-amylase as time-temperature-integrator (TTI).
PubMed: Optimization of alpha-amylase production for the green synthesis of gold nanoparticles.
PubMed: [Comparison between alpha-amylase from B. amyloliquefaciens and B. licheniformis].
PubMed: Kinetic stabilization of Bacillus licheniformis alpha-amylase through introduction of hydrophobic residues at the surface.
PubMed: Kinetic characterization of extracellular alpha-amylase from a derepressed mutant of Bacillus licheniformis.
PubMed: Modification of alpha-amylase from Bacillus licheniformis by the polyaldehyde derived from beta-cyclodextrine and alpha-amylase thermostability.
PubMed: Crystallization and a preliminary X-ray crystallographic study of alpha-amylase from Bacillus licheniformis.
PubMed: Stabilization of Bacillus licheniformis alpha-amylase by specific antibody which recognizes the N-terminal fragment of the enzyme.
PubMed: Enzymatic hydrolysis of soluble starch with an alpha-amylase from Bacillus licheniformis.
PubMed: Thermostable alpha-amylase production by immobilized Bacillus licheniformis cells in agar gel and on acrylonitrile/acrylamide membranes.
PubMed: Cloning in Bacillus subtilis of an extremely thermostable alpha amylase: comparison with other cloned heatstable alpha amylases.
PubMed: Electron microscopic investigation of the diffusion of Bacillus licheniformis alpha-amylase into corn starch granules.
PubMed: Hyperthermostable mutants of Bacillus licheniformis alpha-amylase: thermodynamic studies and structural interpretation.
PubMed: Kinetics of alpha-amylase production in a continuous culture of Bacillus licheniformis.
PubMed: [Comparison of chemical and thermal stability of soluble and immobilized alpha-amylases from B. licheniformis and A. oryzae].
PubMed: Effect of gelatinization and hydrolysis conditions on the selectivity of starch hydrolysis with alpha-amylase from Bacillus licheniformis.
PubMed: Hydrolysis of soluble starch using Bacillus licheniformis alpha-amylase immobilized on superporous CELBEADS.
PubMed: Activity and action pattern of Bacillus licheniformis alpha-amylase in aqueous ethanol.
PubMed: Ca-binding to Bacillus licheniformis alpha-amylase (BLA).
PubMed: Binding of Tris to Bacillus licheniformis alpha-amylase can affect its starch hydrolysis activity.
PubMed: [Effect of monovalent cations on the thermostability of alpha-amylase from Bacillus licheniformis MB-80].
PubMed: Targeted deletion of genes encoding extracellular enzymes in Bacillus licheniformis and the impact on the secretion capability.
PubMed: Enzyme activities and antibiotic susceptibility of colonial variants of Bacillus subtilis and Bacillus licheniformis.
PubMed: Extracellular enzyme synthesis in a sporulation-deficient strain of Bacillus licheniformis.
PubMed: cis sequences involved in modulating expression of Bacillus licheniformis amyL in Bacillus subtilis: effect of sporulation mutations and catabolite repression resistance mutations on expression.
PubMed: Enzymatic properties of a novel liquefying alpha-amylase from an alkaliphilic Bacillus isolate and entire nucleotide and amino acid sequences.
PubMed: Transcriptome profiling of degU expression reveals unexpected regulatory patterns in Bacillus megaterium and discloses new targets for optimizing expression.
PubMed: Cloning and expression of an amylase gene from Bacillus stearothermophilus.
PubMed: Isolation of haemolytic bacilli from field-collected Culicoides oxystoma and Culicoides peregrinus: potential vectors of bluetongue virus in West Bengal, India.
PubMed: Strain development in Bacillus licheniformis: construction of biologically contained mutants deficient in sporulation and DNA repair.
PubMed: Crystallization and preliminary crystallographic study of bacterial alpha-amylases.
PubMed: Why is one Bacillus alpha-amylase more resistant against irreversible thermoinactivation than another?
PubMed: Expression and secretion of an acid-stable alpha-amylase gene in Bacillus Subtilis by SacB promoter and signal peptide.
PubMed: Deduced amino-acid sequence of a calcium-free alpha-amylase from a strain of Bacillus: implications from molecular modeling of high oxidation stability and chelator resistance of the enzyme.
PubMed: Enzymatic degradation products from a marine polysaccharide YCP with different immunological activity and binding affinity to macrophages, hydrolyzed by alpha-amylases from different origins.
PubMed: Crystal structure of calcium-free alpha-amylase from Bacillus sp. strain KSM-K38 (AmyK38) and its sodium ion binding sites.
PubMed: Construction and evaluation of shuttle vector, pGYC4α, based on pYC2 from Lactobacillus sakei.
PubMed: One-step production of immobilized alpha-amylase in recombinant Escherichia coli.
PubMed: Thermostabilization by proline substitution in an alkaline, liquefying alpha-amylase from Bacillus sp. strain KSM-1378.
PubMed: Amino acid residues stabilizing a Bacillus alpha-amylase against irreversible thermoinactivation.
PubMed: Numerical taxonomy of alpha-amylase producing Bacillus species.
PubMed: Characterization of the sacQ genes from Bacillus licheniformis and Bacillus subtilis.
PubMed: Characterisation of mutagenised acid-resistant alpha-amylase expressed in Bacillus subtilis WB600.
PubMed: Integrable alpha-amylase plasmid for generating random transcriptional fusions in Bacillus subtilis.
PubMed: Gene fusions involving the structural gene of B. licheniformis coding for a thermostable alpha-amylase.
PubMed: The influence of protein folding on late stages of the secretion of alpha-amylases from Bacillus subtilis.
PubMed: Enzyme-assisted supercritical carbon dioxide extraction of black pepper oleoresin for enhanced yield of piperine-rich extract.
PubMed: Feed rate control in fed-batch fermentations based on frequency content analysis.
PubMed: Structural analysis of a chimeric bacterial alpha-amylase. High-resolution analysis of native and ligand complexes.
PubMed: Thermostability of soluble and immobilized alpha-amylase from Bacillus licheniformis.
PubMed: Purification and Characterization of alpha-Amylase from Bacillus licheniformis CUMC305.
PubMed: Improved thermostability of a Bacillus alpha-amylase by deletion of an arginine-glycine residue is caused by enhanced calcium binding.
PubMed: Influence of a cell-wall-associated protease on production of alpha-amylase by Bacillus subtilis.
PubMed: Oxygen-transfer strategy and its regulation effects in serine alkaline protease production by Bacillus licheniformis.
PubMed: High pressure, thermal, and combined pressure-temperature stabilities of alpha-amylases from Bacillus species.
PubMed: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase.
PubMed: A dispersion model for predicting the extent of starch liquefaction by Bacillus licheniformis alpha-amylase during reactive extrusion.
PubMed: Effect of temperature on some characteristics of the thermostable α-amylase from Bacillus licheniformis.
PubMed: Topographical and enzymatic characterization of amylases from the extremely thermophilic eubacterium Thermotoga maritima.
PubMed: Thermostability enhancement and change in starch hydrolysis profile of the maltohexaose-forming amylase of Bacillus stearothermophilus US100 strain.
PubMed: Molecular and enzymatic characterization of a maltogenic amylase that hydrolyzes and transglycosylates acarbose.
PubMed: Isolation and molecular characterization of chitinase-deficient Bacillus licheniformis strains capable of deproteinization of shrimp shell waste to obtain highly viscous chitin.
PubMed: Translocation of the precursor of alpha-amylase into Bacillus subtilis membrane vesicles.
PubMed: Expression of Bacillus protease (Protease BYA) from Bacillus sp. Y in Bacillus subtilis and enhancement of its specific activity by site-directed mutagenesis-improvement in productivity of detergent enzyme-.
PubMed: Temperature impacts the multiple attack action of amylases.
PubMed: Cloning, sequencing, and expression of the gene encoding extracellular alpha-amylase from Pyrococcus furiosus and biochemical characterization of the recombinant enzyme.
PubMed: Alcoholysis reactions from starch with alpha-amylases.
PubMed: Recovery and characterization of a 30.7-kDa protein from Bacillus licheniformis associated with inhibitory activity against methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, and Listeria monocytogenes.
PubMed: Replicative and integrative plasmids for production of human interferon gamma in Bacillus subtilis.
PubMed: Nucleotide sequence, structural investigation and homology modeling studies of a Ca2+-independent alpha-amylase with acidic pH-profile.
PubMed: Hyperthermostable variants of a highly thermostable alpha-amylase.
PubMed: A stochastic model for predicting dextrose equivalent and saccharide composition during hydrolysis of starch by alpha-amylase.
PubMed: Occupational asthma caused by bacillary amylase used in the detergent industry.
PubMed: Immobilization of alpha-amylase on poly(vinyl alcohol)-coated perfluoropolymer supports for use in enzyme reactors.
PubMed: Thermostability of irreversible unfolding alpha-amylases analyzed by unfolding kinetics.
PubMed: Crystal structure of Bacillus stearothermophilus alpha-amylase: possible factors determining the thermostability.
PubMed: Comparative studies on trifluoroethanol (TFE) state of a thermophilic alpha-amylase and its mesophilic counterpart: limited proteolysis, conformational analysis, aggregation and reactivation of the enzymes.
PubMed: Screening and identification of polyhydroxyalkanoates producing bacteria and biochemical characterization of their possible application.
PubMed: Electrostatics in the active site of an alpha-amylase.
PubMed: Production of heterologous proteins in Bacillus subtilis: the effect of the joint between signal sequence and mature protein on yield.
PubMed: Pyrococcus furiosus alpha-amylase is stabilized by calcium and zinc.
PubMed: Comparison of the molten globule states of thermophilic and mesophilic alpha-amylases.
PubMed: Protein engineering of alpha-amylase for low pH performance.
PubMed: Thermodynamic and activation parameters for the hydrolysis of amylose with Bacillus alpha-amylases in a diluted anionic surfactant solution.
PubMed: Diversity of culturable bacterial endophytes of saffron in Kashmir, India.
PubMed: [Expression of a thermostable a-amylase mutant into Escherichia coli and Pichia pastoris].
PubMed: Simultaneous biosynthesis and purification of two extracellular Bacillus hydrolases in aqueous two-phase systems.
PubMed: Degradation of the framework of the Chlamydomonas cell wall by proteases present in a commercially available alpha-amylase preparation.
PubMed: [Effect of the changes of amino acids on both signal peptide C-terminal and mature protein N-terminal region to the secretion of alpha-amylase in B. subtilis].
PubMed: Activity and stability of a thermostable alpha-amylase compared to its mesophilic homologue: mechanisms of thermal adaptation.
PubMed: Isolation, characterization and identification of pericarp-degrading bacteria for the production of off-odour-free white pepper from fresh berries of Piper nigrum L.
PubMed: Expression and secretion of alpha-amylase and glucoamylase in Saccharomyces cerevisiae.
PubMed: Isolation and characterization of genetic expression and secretion signals from Enterococcus faecalis through the use of broad-host-range alpha-amylase probe vectors.
PubMed: Direct screening for high-level expression of an introduced alpha-amylase gene in plants.
PubMed: Effect of glycosylation on the catalytic and conformational stability of homologous alpha-amylases.
PubMed: [Construct of the stable-produced strain for the thermostable alpha-amylase].
PubMed: Cell-associated degradation affects the yield of secreted engineered and heterologous proteins in the Bacillus subtilis expression system.
PubMed: Structural stability and unfolding properties of thermostable bacterial alpha-amylases: a comparative study of homologous enzymes.
PubMed: Lactobacillus plantarum amylase acting on crude starch granules. Native isoforms and activity changes after limited proteolysis.
PubMed: Sustained expression of keratinase gene under PxylA and PamyL promoters in the recombinant Bacillus megaterium MS941.
PubMed: [Effect of changes of amino acids of N-terminal region of the mature protein on secretion of alpha-amylase in B. subtilis].
PubMed: The influence of secretory-protein charge on late stages of secretion from the Gram-positive bacterium Bacillus subtilis.
PubMed: [The influence of coordinational germanium compounds on the activity of glycosidases].
PubMed: The alpha-amylase gene amyH of the moderate halophile Halomonas meridiana: cloning and molecular characterization.
PubMed: Thermostable alpha-amylase conjugated antibodies as probes for immunodetection in ELISA.
PubMed: Thermodynamic stability of a cold-active alpha-amylase from the Antarctic bacterium Alteromonas haloplanctis.
PubMed: Recognition sequence specificity of signal peptidase I and the role of signal peptide in secretion of protein in Bacillus subtilis.
PubMed: Close evolutionary relatedness of alpha-amylases from Archaea and plants.
PubMed: Proteolysis of mesophilic and thermophilic alpha-amylases: a comparative study.
PubMed: A method for determination of N-glycosylation sites in glycoproteins by collision-induced dissociation analysis in fast atom bombardment mass spectrometry: identification of the positions of carbohydrate-linked asparagine in recombinant alpha-amylase by treatment with peptide-N-glycosidase F in 18O-labeled water.
PubMed: Poly-extremotolerant bacterium isolated from reverse osmosis reject: an implication toward waste water management.
PubMed: Chemical synthesis of a dual branched malto-decaose: a potential substrate for alpha-amylases.
PubMed: The twin-arginine signal peptide of Bacillus subtilis YwbN can direct either Tat- or Sec-dependent secretion of different cargo proteins: secretion of active subtilisin via the B. subtilis Tat pathway.
PubMed: Hydrolysis of wheat B-starch and characterisation of acetylated maltodextrin.
PubMed: Use of homologous expression-secretion signals and vector-free stable chromosomal integration in engineering of Lactobacillus plantarum for alpha-amylase and levanase expression.
PubMed: Heterologous protein secretion in Lactococcus lactis is enhanced by the Bacillus subtilis chaperone-like protein PrsA.
PubMed: Carbon sources affect metabolic capacities of Bacillus species for the production of industrial enzymes: theoretical analyses for serine and neutral proteases and alpha-amylase.
PubMed: Intrinsic halotolerance of the psychrophilic alpha-amylase from Pseudoalteromonas haloplanktis.
PubMed: High-temperature alkaline alpha-amylase from Bacillus iicheniformis TCRDC-B13.
PubMed: Chemical modification of lysine residues in Bacillus alpha-amylases: effect on activity and stability.
PubMed: Bacillus stearothermophilus neopullulanase selective hydrolysis of amylose to maltose in the presence of amylopectin.
PubMed: Cloning and characterization of the 5' region of the cell wall protein gene operon in Bacillus brevis 47.
PubMed: Rope-producing strains of Bacillus spp. from wheat bread and strategy for their control by lactic acid bacteria.
PubMed: Construction and use of signal sequence selection vectors in Escherichia coli and Bacillus subtilis.
PubMed: A thermostable glucoamylase from Bispora sp. MEY-1 with stability over a broad pH range and significant starch hydrolysis capacity.
PubMed: Characterization of signal-sequence-coding regions selected from the Bacillus subtilis chromosome.
PubMed: Efficiency of transcriptional terminators in Bacillus subtilis.
PubMed: Development of an owoh-type product from African yam beans (Sphenostylis stenocarpa) (Hoechst (ex. A. Rich.) Harms.) seeds by solid substrate fermentation.
PubMed: Influence of the coating formulation on enzymatic digestibility and drug release from 5-aminosalicylic acid pellets coated with mixtures of high-amylose starch and Surelease intended for colon-specific drug delivery.
PubMed: Multiple and tandemly arranged promoters of the cell wall protein gene operon in Bacillus brevis 47.
PubMed: Amylose formulations for drug delivery to the colon: a comparison of two fermentation models to assess colonic targeting performance in vitro.
PubMed: Activity of an enzyme immobilized on superparamagnetic particles in a rotational magnetic field.
PubMed: The effect of process conditions on the alpha-amylolytic hydrolysis of amylopectin potato starch: An experimental design approach.
PubMed: Validation and use of an enzymic time-temperature integrator to monitor thermal impacts inside a solid/liquid model food.
PubMed: A strategy to construct vector-free amylolytic strains through nondisruptive homologous recombination: application to Enterococcus faecalis.
PubMed: Analysis of the conformational transitions of proteins by temperature-gradient gel electrophoresis.
PubMed: Combined use of two single-component enzymatic time-temperature integrators: application to industrial continuous rotary processing of canned ravioli.
PubMed: Construction of high, intermediate and low-copy-number promoter-probe plasmids for Bacillus subtilis.
PubMed: [Enzyme modification by natural chemical chaperons of microorganisms].
PubMed: [Thermophilic bacteria from hot springs of Kamchatka].
PubMed: Concanavalin A- and wheat germ agglutinin-conjugated lectins as a tool for the identification of multiple N-glycosylation sites in heterologous protein expressed in yeast.
PubMed: Purification of thermamylase in multicompartment electrolyzers with isoelectric membranes: the problem of protein solubility.
PubMed: Expression and purification of epidermal cell differentiation inhibitor (EDIN) from Bacillus subtilis.
PubMed: Effect of boiling and roasting on the fermentation of soybeans into dawadawa (soy-dawadawa).
PubMed: Protein export elements from Lactococcus lactis.
PubMed: Surface bio-magnetism on bacterial cells adhesion and surface proteins secretion.
PubMed: Construction and characterization of nisin-controlled expression vectors for use in Lactobacillus reuteri.
PubMed: Effect of temperature on the saccharide composition obtained after alpha-amylolysis of starch
PubMed: The influence of polyalcohols and carbohydrates on the thermostability of alpha-amylase.
PubMed: Hypersensitivity reactions and specific antibodies in workers exposed to industrial enzymes at a biotechnology plant.
PubMed: Study of stability of recombinant plasmids during the continuous culture of Bacillus stearothermophilus NUB3621 in nonselective medium.
PubMed: In vivo genetic engineering: homologous recombination as a tool for plasmid construction.
PubMed: Enzymatic production of glucose from different qualities of grain sorghum and application of ultrasound to enhance the yield.
PubMed: Protein secretion controlled by a synthetic gene in Escherichia coli.
PubMed: Purification, characterization and enzymatic degradation of YCP, a polysaccharide from marine filamentous fungus Phoma herbarum YS4108.
PubMed: Development characterization and use of a high-performance enzymatic time-temperature integrator for the control of sterilization process' impacts.
PubMed: Expression of foreign genes and selection of promoter sequences in Acholeplasma laidlawii.
PubMed: [Construction of an inducible and efficient expression-secretion shuttle vector of B. subtilis].
PubMed: Analysing the pH-dependent properties of proteins using pKa calculations.
PubMed: Taking charge of proteins from neurodegeneration to industrial biotechnology.
PubMed: [Construction of B. thuringiensis shuttle vector and expression of the cry1C gene].
PubMed: Covalent enzyme immobilization on paramagnetic polyacrolein beads.
PubMed: Evaluation of certain food additives and contaminants.
PubMed: Preparation and characterization of novel bioactive peptides responsible for angiotensin I-converting enzyme inhibition from wheat germ.
PubMed: Comparison of Parental and Transgenic Alfalfa Rhizosphere Bacterial Communities Using Biolog GN Metabolic Fingerprinting and Enterobacterial Repetitive Intergenic Consensus Sequence-PCR (ERIC-PCR).
PubMed: Variation in N-linked oligosaccharide structures on heterologous proteins secreted by the methylotrophic yeast Pichia pastoris.
PubMed: Improved cloning vectors for Bifidobacterium spp.
PubMed: A food-grade expression/secretion vector for Lactococcus lactis that uses an alpha-galactosidase gene as a selection marker.
PubMed: Establishment of a host-vector system in Lactobacillus helveticus with beta-galactosidase activity as a selection marker.
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