serine proteinase from nocardiopsis prasina expressed in bacillus licheniformis
 
Notes:
peptide hydrolases that contain at the active site a serine residue involved in catalysis.
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CAS Number: 37259-58-8 
Category: food additive
 
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Physical Properties:
Assay: 95.00 to 100.00 % 
Food Chemicals Codex Listed: No
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Organoleptic Properties:
 
Odor and/or flavor descriptions from others (if found).
 
 
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Cosmetic Information:
None found
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Suppliers:
Novozymes
iZyme ® B
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Safety Information:
 
Hazards identification
 
Classification of the substance or mixture
GHS Classification in accordance with 29 CFR 1910 (OSHA HCS)
None found.
GHS Label elements, including precautionary statements
 
Pictogram
 
Hazard statement(s)
None found.
Precautionary statement(s)
None found.
Oral/Parenteral Toxicity:
intraperitoneal-mouse LD50  64100 ug/kg
LUNGS, THORAX, OR RESPIRATION: DYSPNEA GASTROINTESTINAL: ULCERATION OR BLEEDING FROM LARGE INTESTINE GASTROINTESTINAL: ULCERATION OR BLEEDING FROM SMALL INTESTINE
Kiso to Rinsho. Clinical Report. Vol. 8, Pg. 2731, 1974.

oral-mouse LD50  15900 mg/kg
SKIN AND APPENDAGES (SKIN): HAIR: OTHER SENSE ORGANS AND SPECIAL SENSES: PTOSIS: EYE BEHAVIORAL: SOMNOLENCE (GENERAL DEPRESSED ACTIVITY)
Kiso to Rinsho. Clinical Report. Vol. 8, Pg. 2731, 1974.

intraperitoneal-rat LD50  62900 ug/kg
GASTROINTESTINAL: ULCERATION OR BLEEDING FROM SMALL INTESTINE BEHAVIORAL: SOMNOLENCE (GENERAL DEPRESSED ACTIVITY) GASTROINTESTINAL: ULCERATION OR BLEEDING FROM LARGE INTESTINE
Kiso to Rinsho. Clinical Report. Vol. 8, Pg. 2731, 1974.

oral-rat LD50  14400 mg/kg
SKIN AND APPENDAGES (SKIN): HAIR: OTHER SENSE ORGANS AND SPECIAL SENSES: PTOSIS: EYE BEHAVIORAL: SOMNOLENCE (GENERAL DEPRESSED ACTIVITY)
Kiso to Rinsho. Clinical Report. Vol. 8, Pg. 2731, 1974.

Dermal Toxicity:
subcutaneous-mouse LD50 110 mg/kg
BEHAVIORAL: SOMNOLENCE (GENERAL DEPRESSED ACTIVITY) SENSE ORGANS AND SPECIAL SENSES: PTOSIS: EYE SKIN AND APPENDAGES (SKIN): "DERMATITIS, OTHER: AFTER SYSTEMIC EXPOSURE"
Kiso to Rinsho. Clinical Report. Vol. 8, Pg. 2731, 1974.

subcutaneous-rat LD50 82900 ug/kg
BEHAVIORAL: SOMNOLENCE (GENERAL DEPRESSED ACTIVITY) SENSE ORGANS AND SPECIAL SENSES: PTOSIS: EYE SKIN AND APPENDAGES (SKIN): "DERMATITIS, OTHER: AFTER SYSTEMIC EXPOSURE"
Kiso to Rinsho. Clinical Report. Vol. 8, Pg. 2731, 1974.

Inhalation Toxicity:
Not determined
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Safety in Use Information:
Category: food additive
Recommendation for serine proteinase from nocardiopsis prasina expressed in bacillus licheniformis usage levels up to:
 not for fragrance use.
 
Recommendation for serine proteinase from nocardiopsis prasina expressed in bacillus licheniformis flavor usage levels up to:
 not for flavor use.
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Safety References:
ClinicalTrials.gov: search
AIDS Citations: Search
Cancer Citations: Search
Toxicology Citations: Search
EPA ACToR: Toxicology Data
EPA Substance Registry Services (SRS): Registry
National Institute of Allergy and Infectious Diseases: Data
Chemidplus: 0037259588
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References:
Pubchem (sid): 135291066
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Other Information:
HMDB (The Human Metabolome Database): Search
FDA Listing of Food Additive Status: View
VCF-Online: VCF Volatile Compounds in Food
Wikipedia: View
Wikipedia2: View2
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Potential Blenders and core components note
 
None Found
 
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Potential Uses:
None Found
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Occurrence (nature, food, other): note
 bacillus licheniformis
Search  PMC Picture
 nocardiopsis prasina
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 serine proteinase
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Synonyms:
 iZyme B
 microbial chymotrypsin
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Articles:
PubMed: Isolation and characterization of an ovoinhibitor, a multidomain Kazal-like inhibitor from Turkey (Meleagris gallopavo) seminal plasma.
PubMed: Genetic and biochemical evidence that recombinant Enterococcus spp. strains expressing gelatinase (GelE) produce bovine milk-derived hydrolysates with high angiotensin converting enzyme-inhibitory activity (ACE-IA).
PubMed: Purification, characterization, cDNA cloning and in vitro expression of a serine proteinase from the intestinal tract of sea cucumber (Stichopus japonicus) with collagen degradation activity.
PubMed: Antitumor activity of the protein and small molecule component fractions from Agrocybe aegerita through enhancement of cytokine production.
PubMed: Characterization of carp seminal plasma proteome in relation to blood plasma.
PubMed: New structural variants of aeruginosin produced by the toxic bloom forming cyanobacterium Nodularia spumigena.
PubMed: Purification of a Kunitz-type inhibitor from Acacia polyphyllaDC seeds: characterization and insecticidal properties against Anagasta kuehniella Zeller (Lepidoptera: Pyralidae).
PubMed: What are the proteolytic enzymes of honey and what they do tell us? A fingerprint analysis by 2-D zymography of unifloral honeys.
PubMed: Inactivation of the serine proteinase operon (proMCD) of Staphylococcus warneri M: serine proteinase and cysteine proteases are involved in the autolysis.
PubMed: Wheat Subtilisin/Chymotrypsin Inhibitor (WSCI) as a scaffold for novel serine protease inhibitors with a given specificity.
PubMed: Molecular characterization and structural instability of the industrially important composite metabolic plasmid pLP712.
PubMed: Purification and characterization of a proteinase from the probiotic Lactobacillus rhamnosus OXY.
PubMed: Synthesis and inhibitory activities against colon cancer cell growth and proteasome of alkylresorcinols.
PubMed: Anti-inflammatory and neuroactive properties of selected fruit extracts.
PubMed: Telaprevir and boceprevir in African Americans with genotype 1 chronic hepatitis C: implications for patients and providers.
PubMed: Interaction of protein C inhibitor with the type II transmembrane serine protease enteropeptidase.
PubMed: Chickpea (Cicer arietinum) and other plant-derived protease inhibitor concentrates inhibit breast and prostate cancer cell proliferation in vitro.
PubMed: SERINE proteinase like activity in apolipophorin III from the hemolymph of desert locust, Schistocerca gregaria.
PubMed: Identification of the second form of acrosin in Turkey spermatozoa.
PubMed: Mutation analysis of barley malt protein Z4 and protein Z7 on beer foam stability.
PubMed: Different protein expression profiles in cheese and clinical isolates of Enterococcus faecalis revealed by proteomic analysis.
PubMed: Evaluation of certain food additives.
PubMed: Purification, characterization and cDNA cloning of a trypsin from the hepatopancreas of snakehead (Channa argus).
PubMed: 24kDa Trypsin: A predominant protease purified from the viscera of hybrid catfish (Clarias macrocephalus×Clarias gariepinus).
PubMed: Antimicrobial potential of egg yolk ovoinhibitor, a multidomain Kazal-like inhibitor of chicken egg.
PubMed: The mechanisms by which polyamines accelerate tumor spread.
PubMed: Polyphenol fatty acid esters as serine protease inhibitors: a quantum-chemical QSAR analysis.
PubMed: Evidence of cell-associated proteinases from Virgibacillus sp. SK33 isolated from fish sauce fermentation.
PubMed: A kinetic study on sesame cake protein hydrolysis by Alcalase.
PubMed: Central vaspin administration acutely reduces food intake and has sustained blood glucose-lowering effects.
PubMed: Activity-based protein profiling of organophosphorus and thiocarbamate pesticides reveals multiple serine hydrolase targets in mouse brain.
PubMed: Characterization of a glutenin-specific serine proteinase of Sunn bug Eurygaster integricepts Put.
PubMed: Mung bean trypsin inhibitor is effective in suppressing the degradation of myofibrillar proteins in the skeletal muscle of blue scad (Decapterus maruadsi).
PubMed: Inhibition of plasma hyaluronan-binding protein autoactivation by laccaic acid.
PubMed: Expression of diverse midgut serine proteinases in the sericigenous Lepidoptera Antheraea assamensis (Helfer) is influenced by choice of host plant species.
PubMed: Comparative analysis of proteome maps of silkworm hemolymph during different developmental stages.
PubMed: Curious about pesticide action.
PubMed: Isolation, characterization and cDNA sequencing of acrosin from turkey spermatozoa.
PubMed: Effects of the probiotic, Bacillus subtilis E20, on the survival, development, stress tolerance, and immune status of white shrimp, Litopenaeus vannamei larvae.
PubMed: Purification and characterization of a halotolerant serine proteinase from thermotolerant Bacillus licheniformis RKK-04 isolated from Thai fish sauce.
PubMed: Purification and characterization of a collagenolytic serine proteinase from the skeletal muscle of red sea bream (Pagrus major).
PubMed: The cytotoxic effect of Bowman-Birk isoinhibitors, IBB1 and IBBD2, from soybean (Glycine max) on HT29 human colorectal cancer cells is related to their intrinsic ability to inhibit serine proteases.
PubMed: Differential proteomic analysis of Clostridium perfringens ATCC13124; identification of dominant, surface and structure associated proteins.
PubMed: The Kunitz-like modulatory protein haemangin is vital for hard tick blood-feeding success.
PubMed: Interactions of alpha1-proteinase inhibitor with small ligands of therapeutic potential: binding with retinoic acid.
PubMed: Glucose-6-phosphate isomerase is an endogenous inhibitor to myofibril-bound serine proteinase of crucian carp (Carassius auratus).
PubMed: Discovery of a novel proteasome inhibitor selective for cancer cells over non-transformed cells.
PubMed: Aflatoxin B1 misregulates the activity of serine proteases: possible implications in the toxicity of some mycotoxin.
PubMed: Factors affecting rheological characteristics of fibril gels: the case of beta-lactoglobulin and alpha-lactalbumin.
PubMed: Two different proteases from Streptomyces hygroscopicus are involved in transglutaminase activation.
PubMed: A set of serine proteinase paralogs are required for blood-digestion in the ixodid tick Haemaphysalis longicornis.
PubMed: Characterization of a serine proteinase mediating encystation of Acanthamoeba.
PubMed: Serratia entomophila inoculation causes a defect in exocytosis in Costelytra zealandica larvae.
PubMed: Indicain, a dimeric serine protease from Morus indica cv. K2.
PubMed: Molecular and immunological characterization of a wheat serine proteinase inhibitor as a novel allergen in baker's asthma.
PubMed: Isolation, characterization and cDNA sequencing of a Kazal family proteinase inhibitor from seminal plasma of turkey (Meleagris gallopavo).
PubMed: Autolysis of Pacific white shrimp (Litopenaeus vannamei) meat: characterization and the effects of protein additives.
PubMed: Liver prenylated methylated protein methyl esterase is the same enzyme as Sus scrofa carboxylesterase.
PubMed: Roles of functional and structural domains of hepatocyte growth factor activator inhibitor type 1 in the inhibition of matriptase.
PubMed: Effect of a Bowman-Birk proteinase inhibitor from Phaseolus coccineus on Hypothenemus hampei gut proteinases in vitro.
PubMed: NaCl-activated extracellular proteinase from Virgibacillus sp. SK37 isolated from fish sauce fermentation.
PubMed: Sulfated chitooligosaccharides as prolyl endopeptidase inhibitor.
PubMed: Neutrophil interaction with the hemostatic system contributes to liver injury in rats cotreated with lipopolysaccharide and ranitidine.
PubMed: Kinetics of the inhibition of fusarium serine proteinases by barley (Hordeum vulgare L.) inhibitors.
PubMed: Molecular and reverse genetic characterization of serine proteinase-induced hemolysis in the midgut of the ixodid tick Haemaphysalis longicornis.
PubMed: Purification, characterization, and cDNA cloning of a myofibril-bound serine proteinase from the skeletal muscle of crucian carp (Carassius auratus).
PubMed: The critical role of aprotinin in controlling haemostasis in conjunction with non-pharmacological blood-saving strategies during routine coronary artery bypass surgery.
PubMed: Specificity of an extracellular proteinase from Conidiobolus coronatus and its inhibition by an inhibitor from insect hemolymph.
PubMed: Serine proteases and metalloproteases associated with pathogenesis but not host specificity in the Entomophthoralean fungus Zoophthora radicans.
PubMed: Purification and characterization of an alkaline serine protease producing angiotensin I-converting enzyme inhibitory peptide from Bacillus sp. SS103.
PubMed: Review and application of serine protease inhibition in coronary artery bypass graft surgery.
PubMed: Extracellular prolyl endoprotease from Aspergillus niger and its use in the debittering of protein hydrolysates.
PubMed: Structural and functional study of an Anemonia elastase inhibitor, a "nonclassical" Kazal-type inhibitor from Anemonia sulcata.
PubMed: Attenuation of skeletal muscle atrophy via protease inhibition.
PubMed: Evidence-based review of the role of aprotinin in blood conservation during orthopaedic surgery.
PubMed: Conformational stability of the potato serine protease inhibitor group.
PubMed: Gelatinases and serine proteinase inhibitors of seminal plasma and the reproductive tract of turkey (Meleagris gallopavo).
PubMed: Protease B from Debaryomyces hansenii: purification and biochemical properties.
PubMed: Tentative assignment of the potato serine protease inhibitor group as beta-II proteins based on their spectroscopic characteristics.
PubMed: Purification and characterization of intracellular proteinase from Lactobacillus casei ssp. casei LLG.
PubMed: Regulation of gelatinases expression by cytokines, endotoxin, and pharmacological agents in the human osteoarthritic knee.
PubMed: Redesign of catalytic center of an enzyme: aspartic to serine proteinase.
PubMed: Effects of native and denatured whey proteins on plasminogen activator activity.
PubMed: Insights into the reaction mechanism of the coagulation of soy protein isolates induced by subtilisin carlsberg.
PubMed: Screening for peptides from fish and cheese inhibitory to prolyl endopeptidase.
PubMed: Isolation, characterization, and cDNA sequencing of alpha-1-antiproteinase-like protein from rainbow trout seminal plasma.
PubMed: Therapeutic efficacy of once-daily oral administration of a Kunitz-type protease inhibitor, bikunin, in a mouse model and in human cancer.
PubMed: Molecular mechanism of enzyme inhibition: prediction of the three-dimensional structure of the dimeric trypsin inhibitor from Leucaena leucocephala by homology modelling.
PubMed: A prolyl endopeptidase-inhibiting antioxidant from Phyllanthus ussurensis.
PubMed: Trypsin/alpha-amylase inhibitors inactivate the endogenous barley/malt serine endoproteinase SEP-1.
PubMed: Expression of enteropeptidase in differentiated enterocytes, goblet cells, and the tumor cells in human duodenum.
PubMed: The most abundant protease inhibitor in potato tuber (cv. Elkana) is a serine protease inhibitor from the Kunitz family.
PubMed: Crystal structure of S-ovalbumin as a non-loop-inserted thermostabilized serpin form.
PubMed: Analysis of molecular interactions in heat-induced aggregation of a non-inhibitory serpin ovalbumin using a molecular chaperone.
PubMed: Dietary regulation of serine proteinases that are resistant to serine proteinase inhibitors.
PubMed: Purification and characterization of heat-stable alkaline proteinase from bigeye snapper (Priacanthus macracanthus) muscle.
PubMed: Purification and identification of barley (Hordeum vulgare L.) proteins that inhibit the alkaline serine proteinases of Fusarium culmorum.
PubMed: Inhibitory specificity change of the ovomucoid third domain of the silver pheasant upon introduction of an engineered Cys14-Cys39 bond.
PubMed: Thiol-activated serine proteinases from nymphal hemolymph of the African migratory locust, Locusta migratoria migratorioides.
PubMed: Purification and characterization of collagenolytic proteases from the hepatopancreas of northern shrimp (Pandalus eous).
PubMed: Green tea polyphenols inhibit metalloproteinase activities in the skin, muscle, and blood of rainbow trout.
PubMed: Neuroendocrine control of intestinal mucosal mast cells under physiological conditions.
PubMed: Tick-Encoded serine proteinase inhibitors (serpins); potential target antigens for tick vaccine development.
PubMed: Identification of a serine protease inhibitor homologue in Bird's Nest by an integrated proteomics approach.
PubMed: Acrosin activity in turkey spermatozoa: assay by clinical method and effect of zinc and benzamidine on the activity.
PubMed: Fas aggregation does not correlate with Fas-mediated apoptosis.
PubMed: Increased concentration of fecal alpha1-antitrypsin is associated with cow's milk allergy in infants with atopic eczema.
PubMed: Pernin: a novel, self-aggregating haemolymph protein from the New Zealand green-lipped mussel, Perna canaliculus (Bivalvia: Mytilidae).
PubMed: Purification and Characterization of a Tripeptidase from Lactobacillus sake.
PubMed: Purification and characterization of a cystatin from the leaves of methyl jasmonate treated tomato plants.
PubMed: Characteristics of sperm acrosin-like activity of paddlefish (Polyodon spathula Walbaum).
PubMed: The structure of an insect chymotrypsin.
PubMed: Induction of atherosclerosis in Brown Norway rats by immunization with ovalbumin.
PubMed: Structural determinants of the bifunctional corn Hageman factor inhibitor: x-ray crystal structure at 1.95 A resolution.
PubMed: [Reproductive and developmental toxicity study of sodium N-[2-[4-(2,2-dimethylpropionyloxy) phenylsulfonylamino] benzoyl] aminoacetate tetrahydrate (ONO-5046.Na) (3). Teratogenicity study in rabbits].
PubMed: [Reproductive and developmental toxicity study of sodium N-[2-[4-(2,2-dimethylpropionyloxy) phenylsulfonylamino] benzoyl] aminoacetate tetrahydrate (ONO-5046.Na) (2). Study for effects on pre- and postnatal development in rats, including maternal function].
PubMed: [Reproductive and developmental toxicity study of sodium N-[2-[4-(2,2-dimethylpropionyloxy) phenylsulfonylamino] benzoyl] aminoacetate tetrahydrate (ONO-5046.Na (1). Fertility study in rats].
PubMed: [Toxicity study of sodium N-[2-[4-(2,2-dimethylpropionyloxy) phenylsulfonylamino] benzoyl] aminoacetate tetrahydrate (ONO-5046.Na) (5). 6-month repeated dose intravenous toxicity study in dogs with 1-month recovery test].
PubMed: [Toxicity study of sodium N-[2-[4-(2,2-dimethylpropionyloxy) phenylsulfonylamino] benzoyl] aminoacetate tetrahydrate (ONO-5046.Na) (4). 6-month repeated dose intravenous toxicity study in rats with 1-month recovery test].
PubMed: [Toxicity study of sodium N-[2-[4-(2,2-dimethylpropionyloxy) phenylsulfonylamino] benzoyl] aminoacetate tetrahydrate (ONO-5046.Na) (3). 4-week repeated dose intravenous toxicity study in dogs with 4-week recovery test].
PubMed: [Toxicity study of sodium N-[2-[4-(2,2-dimethylpropionyloxy) phenylsulfonylamino] benzoyl] aminoacetate tetrahydrate (ONO-5046.Na) (2). 4-week repeated dose intravenous toxicity study in rats with 4-week recovery test].
PubMed: [Toxicity study of sodium N-[2-[4-(2,2-dimethylpropionyloxy) phenylsulfonylamino] benzoyl] aminoacetate tetrahydrate (ONO-5046.Na) (1). Single-dose intravenous toxicity studies in rats and dogs].
PubMed: [Intraepithelial T cell population in ileum from ovalbumin sensitized rabbit].
PubMed: Characterization of major midgut proteinase cDNAs from Helicoverpa armigera larvae and changes in gene expression in response to four proteinase inhibitors in the diet.
PubMed: Antigenic determinants and reactive sites of a trypsin/chymotrypsin double-headed inhibitor from horse gram (Dolichos biflorus).
PubMed: Presence in bovine milk of two protease inhibitors of the plasmin system.
PubMed: Production of an extracellular trypsin-like protease by the fungal plant pathogen Verticillium dahliae.
PubMed: Accelerated production of dry fermented sausage.
PubMed: Production of chicken ovalbumin in Escherichia coli.
PubMed: Metabolism of enterostatin in rat intestine, brain membranes, and serum: differential involvement of proline-specific peptidases.
PubMed: Evolution of the proteinase inhibitor I family and apparent lack of hypervariability in the proteinase contact loop.
PubMed: Purification and characterization of alkaline serine protease from an alkalophilic Streptomyces sp.
PubMed: Effects of serine protease inhibitor, TAME, on IL-1 beta in LPS-stimulated human monocytes: relationship between synthesis and release of a 33-kDa precursor and the 17-kDa biologically active species.
PubMed: Molecular cloning of silkworm (Bombyx mori) antichymotrypsin. A new member of the serpin superfamily of proteins from insects.
PubMed: Purification and characterization of an alkaline proteinase from kidney cortex lysosomes.
PubMed: Improved survival in mice with diet-induced pancreatitis treated with new potent protease inhibitor, E-3123 and a broad spectrum antibiotic, cefmetazole.
PubMed: Patchwork-structure serpins from silkworm (Bombyx mori) larval hemolymph.
PubMed: Production of proteases by psychrotrophic microorganisms.
PubMed: Purification and characterization of a subtilisin inhibitor from seeds of foxtail millet, Setaria italica.
PubMed: Alkaline serine proteinase from Thermomonospora fusca YX. Stability to heat and denaturants.
PubMed: Heat stable proteinase from Thermomonospora fusca. Characterization as a serine proteinase.
PubMed: Limited proteolysis of silkworm antitrypsin by several proteinases.
PubMed: Evidence for the occurrence of an alkaline proteinase in kidney cortex lysosomes.
PubMed: The cell-bound proteinase system of Lactobacillus casei--purification and characterization.
PubMed: A novel fibrinolytic enzyme (nattokinase) in the vegetable cheese Natto; a typical and popular soybean food in the Japanese diet.
PubMed: Purification and characterization of the heat-stable serine proteinase from Thermomonospora fusca YX.
PubMed: Specificities of extracellular and ribosomal serine proteinases from Bacillus natto, a food microorganism.
PubMed: Inhibition of human sperm penetration into zona-free hamster oocytes by proteinase inhibitors.
PubMed: Stability of enzyme inhibitors and lectins in foods and the influence of specific binding interactions.
PubMed: The formation of gamma-caseins during cooling of raw milk.
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