apoaequorin

Articles:

PubMed:	Monitoring yeast intracellular Ca2+ levels using an in vivo bioluminescence assay.
PubMed:	Comparative analysis of MAMP-induced calcium influx in Arabidopsis seedlings and protoplasts.
PubMed:	Calcium dependence of eugenol tolerance and toxicity in Saccharomyces cerevisiae.
PubMed:	Safety assessment of the calcium-binding protein, apoaequorin, expressed by Escherichia coli.
PubMed:	Pretreatment with apoaequorin protects hippocampal CA1 neurons from oxygen-glucose deprivation.
PubMed:	Reconstitution of holo-aequorin with apoaequorin mRNA and coelenterazine in zebrafish embryos.
PubMed:	Stabilisation of recombinant aequorin by polyols: activity, thermostability and limited proteolysis.
PubMed:	Safety assessment of Apoaequorin, a protein preparation: subchronic toxicity study in rats.
PubMed:	A biogenic amine and a neuropeptide act identically: tyramine signals through calcium in Drosophila tubule stellate cells.
PubMed:	In vivo functional calcium imaging of induced or spontaneous activity in the fly brain using a GFP-apoaequorin-based bioluminescent approach.
PubMed:	ALSUntangled no. 18: apoaequorin (Prevagen).
PubMed:	Cch1p mediates Ca2+ influx to protect Saccharomyces cerevisiae against eugenol toxicity.
PubMed:	Cytosolic Ca(2+) determinations in studying plant responses to salinity and oxidative stress.
PubMed:	A yeast expression system for functional and pharmacological studies of the malaria parasite Ca²⁺/H⁺ antiporter.
PubMed:	A stress-specific calcium signature regulating an ozone-responsive gene expression network in Arabidopsis.
PubMed:	Calcium signatures and signaling in cytosol and organelles of tobacco cells induced by plant defense elicitors.
PubMed:	TAT-mediated aequorin transduction: an alternative approach for effective calcium measurements in plant cells.
PubMed:	Visualization, characterization and modulation of calcium signaling during the development of slow muscle cells in intact zebrafish embryos.
PubMed:	Cytosolic calcium rises and related events in ergosterol-treated Nicotiana cells.
PubMed:	Enhanced intracellular Ca2+ concentrations in Escherichia coli and Bacillus subtilis after addition of oligosaccharide elicitors.
PubMed:	Negative feedback regulation of microbe-associated molecular pattern-induced cytosolic Ca2+ transients by protein phosphorylation.
PubMed:	The use of aequorins to record and visualize Ca(2+) dynamics: from subcellular microdomains to whole organisms.
PubMed:	Free Ca2+ as an early intracellular biomarker of exposure of cyanobacteria to environmental pollution.
PubMed:	Application of new semisynthetic aequorins with long half-decay time of luminescence to G-protein-coupled receptor assay.
PubMed:	A peptidomics strategy for discovering endogenous bioactive peptides.
PubMed:	Screening for unknown mutations by a bioluminescent protein truncation test with homogeneous detection.
PubMed:	Intracellular Ca2+ stores could participate to abscisic acid-induced depolarization and stomatal closure in Arabidopsis thaliana.
PubMed:	Mouse ChemR23 is expressed in dendritic cell subsets and macrophages, and mediates an anti-inflammatory activity of chemerin in a lung disease model.
PubMed:	Plant volatiles regulate the activities of Ca2+ -permeable channels and promote cytoplasmic calcium transients in Arabidopsis leaf cells.
PubMed:	Evidence for calcium-mediated perception of plant symbiotic signals in aequorin-expressing Mesorhizobium loti.
PubMed:	Nitric oxide triggers specific and dose-dependent cytosolic calcium transients in Arabidopsis.
PubMed:	Reconstitution of blue fluorescent protein from recombinant apoaequorin and synthetic coelenteramide.
PubMed:	Critical consideration on the relationship between auxin transport and calcium transients in gravity perception of Arabidopsis seedlings.
PubMed:	The intrinsic fluorescence of apo-obelin and apo-aequorin and use of its quenching to characterize coelenterazine binding.
PubMed:	Aequorin variants with improved bioluminescence properties.
PubMed:	Role of calcium in acclimation of the cyanobacterium Synechococcus elongatus PCC 7942 to nitrogen starvation.
PubMed:	An early Ca2+ influx is a prerequisite to thaxtomin A-induced cell death in Arabidopsis thaliana cells.
PubMed:	Soluble protein expression in E. coli cells using IgG-binding domain of protein A as a solubilizing partner in the cold induced system.
PubMed:	Role of neutrophil proteinase 3 and mast cell chymase in chemerin proteolytic regulation.
PubMed:	Purinergic-mediated Ca2+ influx in Dictyostelium discoideum.
PubMed:	Desensitization and internalization of the human motilin receptor is independent of the C-terminal tail.
PubMed:	Infrared reflection-absorption spectroscopy and polarization-modulated infrared reflection-absorption spectroscopy studies of the aequorin langmuir monolayer.
PubMed:	Cloning, expression, purification and characterization of an isotype of clytin, a calcium-binding photoprotein from the luminous hydromedusa Clytia gregarium.
PubMed:	Differences in motilin receptor desensitization after stimulation with motilin or motilides are due to alternative receptor trafficking.
PubMed:	Cytoplasmic calcium increases in response to changes in the gravity vector in hypocotyls and petioles of Arabidopsis seedlings.
PubMed:	Bioluminescence immunoassay for angiotensin II using aequorin as a label.
PubMed:	Determination of affinity and activity of ligands at the human neuropeptide Y Y4 receptor by flow cytometry and aequorin luminescence.
PubMed:	Aequorin luminescence-based assay for 5-hydroxytryptamine (serotonin) type 3 receptor characterization.
PubMed:	Cytosolic Ca2+ regulates protein expression in E. coli through release from inclusion bodies.
PubMed:	Surface properties of "jellyfish": Langmuir monolayer and Langmuir-Blodgett film studies of recombinant aequorin.
PubMed:	Identification of the functional expression of adenosine A3 receptor in pancreas using transgenic mice expressing jellyfish apoaequorin.
PubMed:	Imidazole-assisted catalysis of luminescence reaction in blue fluorescent protein from the photoprotein aequorin.
PubMed:	Ca2+ transient induced by extracellular changes in osmotic pressure in Arabidopsis leaves: differential involvement of cell wall-plasma membrane adhesion.
PubMed:	Fluorescence- and luminescence-based methods for the determination of affinity and activity of neuropeptide Y2 receptor ligands.
PubMed:	Transient expression of apoaequorin in zebrafish embryos: extending the ability to image calcium transients during later stages of development.
PubMed:	Mechanisms of nitric-oxide-induced increase of free cytosolic Ca2+ concentration in Nicotiana plumbaginifolia cells.
PubMed:	[D-1-Nal4]endomorphin-2 is a potent micro-opioid receptor antagonist in the aequorin luminescence-based calcium assay.
PubMed:	Primary evidence for involvement of IP3 in heat-shock signal transduction in Arabidopsis.
PubMed:	Blue fluorescent protein from the calcium-sensitive photoprotein aequorin: catalytic properties for the oxidation of coelenterazine as an oxygenase.
PubMed:	Copper elicits an increase in cytosolic free calcium in cultured tobacco cells.
PubMed:	Proteinaceous and oligosaccharidic elicitors induce different calcium signatures in the nucleus of tobacco cells.
PubMed:	Mitochondrial biogenesis in mtDNA-depleted cells involves a Ca2+-dependent pathway and a reduced mitochondrial protein import.
PubMed:	Comparison of antagonist activity of spantide family at human neurokinin receptors measured by aequorin luminescence-based functional calcium assay.
PubMed:	AtGLR3.4, a glutamate receptor channel-like gene is sensitive to touch and cold.
PubMed:	Cell cycle dependence of elicitor-induced signal transduction in tobacco BY-2 cells.
PubMed:	Blue fluorescent protein from the calcium-sensitive photoprotein aequorin is a heat resistant enzyme, catalyzing the oxidation of coelenterazine.
PubMed:	Evidence of a novel cell signaling role for extracellular adenosine triphosphates and diphosphates in Arabidopsis.
PubMed:	Substitution of conserved glycine residue by alanine in natural and synthetic neuropeptide ligands causes partial agonism at the stomoxytachykinin receptor.
PubMed:	Analysis of nitric oxide signaling functions in tobacco cells challenged by the elicitor cryptogein.
PubMed:	Analysis of the transient increase in cytosolic Ca2+ during the action potential of higher plants with high temporal resolution: requirement of Ca2+ transients for induction of jasmonic acid biosynthesis and PINII gene expression.
PubMed:	Identification of putative voltage-dependent Ca2+-permeable channels involved in cryptogein-induced Ca2+ transients and defense responses in tobacco BY-2 cells.
PubMed:	Circadian and diurnal calcium oscillations encode photoperiodic information in Arabidopsis.
PubMed:	Chromatography of isoforms of recombinant apoaequorin and method for the preparation of aequorin.
PubMed:	One-step purification and refolding of recombinant photoprotein aequorin by immobilized metal-ion affinity chromatography.
PubMed:	Recombinant aequorin as a reporter for receptor-mediated changes of intracellular Ca2+ -levels in Drosophila S2 cells.
PubMed:	Assay for intracellular calcium using a codon-optimized aequorin.
PubMed:	Analysis and effects of cytosolic free calcium increases in response to elicitors in Nicotiana plumbaginifolia cells.
PubMed:	Signal transduction leading to low-temperature tolerance in Arabidopsis thaliana.
PubMed:	Bacterial expression of in vivo-biotinylated aequorin for direct application to bioluminometric hybridization assays.
PubMed:	The motilin pharmacophore in CHO cells expressing the human motilin receptor.
PubMed:	Phospholipase C is required for glucose-induced calcium influx in budding yeast.
PubMed:	Adaptation of aequorin functional assay to high throughput screening.
PubMed:	Bioluminescence immunoassay for thyroxine employing genetically engineered mutant aequorins containing unique cysteine residues.
PubMed:	The lipopolysaccharides of the phytopathogen Xanthomonas campestris pv. campestris induce an oxidative burst reaction in cell cultures of Nicotiana tabacum.
PubMed:	Using epitope-aequorin conjugate recognition in immunoassays for complex proteins.
PubMed:	Differential mRNA degradation of two beta-tubulin isoforms correlates with cytosolic Ca2+ changes in glucan-elicited soybean cells.
PubMed:	Functional characterization of a human receptor for neuropeptide FF and related peptides.
PubMed:	A high-throughput glow-type aequorin assay for measuring receptor-mediated changes in intracellular calcium levels.
PubMed:	Receptor-mediated increase in cytoplasmic free calcium required for activation of pathogen defense in parsley.
PubMed:	The crystal structure of the photoprotein aequorin at 2.3 A resolution.
PubMed:	Use of recombinant aequorin to study calcium homeostasis and monitor calcium transients in response to heat and cold shock in cyanobacteria.
PubMed:	Apoaequorin monitors degradation of endoplasmic reticulum (ER) proteins initiated by loss of ER Ca(2+).
PubMed:	Signal amplification system for DNA hybridization assays based on in vitro expression of a DNA label encoding apoaequorin.
PubMed:	An automated aequorin luminescence-based functional calcium assay for G-protein-coupled receptors.
PubMed:	A serpentine receptor-dependent, Gbeta- and Ca(2+) influx-independent pathway regulates mitogen-activated protein kinase ERK2 in Dictyostelium.
PubMed:	Slow changes in cytosolic free Ca2+ in Escherichia coli highlight two putative influx mechanisms in response to changes in extracellular calcium.
PubMed:	The in situ regeneration and extraction of recombinant aequorin from Escherichia coli cells and the purification of extracted aequorin.
PubMed:	Role of calreticulin in regulating intracellular Ca2+ storage and capacitative Ca2+ entry in HeLa cells.
PubMed:	Recombinant human G protein-coupled lysophosphatidic acid receptors mediate intracellular calcium mobilization.
PubMed:	Salicylic acid induces a cytosolic Ca2+ elevation in yeast.
PubMed:	Aequorea victoria bioluminescence moves into an exciting new era.
PubMed:	Mastoparan induces an increase in cytosolic calcium ion concentration and subsequent activation of protein kinases in tobacco suspension culture cells.
PubMed:	Reduced Ca2+ uptake by mitochondria in pyruvate dehydrogenase-deficient human diploid fibroblasts.
PubMed:	Expression of apo-aequorin during embryonic development; how much is needed for calcium imaging?
PubMed:	A bioluminescent assay for agonist activity at potentially any G-protein-coupled receptor.
PubMed:	The use of Renilla luciferase, Oplophorus luciferase, and apoaequorin as bioluminescent reporter protein in the presence of coelenterazine analogues as substrate.
PubMed:	Hypoosmotic Shock Induces Increases in Cytosolic Ca2+ in Tobacco Suspension-Culture Cells.
PubMed:	Measurement of changes in sarcoplasmic reticulum [Ca2+] in rat tail artery with targeted apoaequorin delivered by an adenoviral vector.
PubMed:	A slow sustained increase in cytosolic Ca2+ levels mediates stalk gene induction by differentiation inducing factor in Dictyostelium.
PubMed:	Regeneration and luminescence of aequorin in Chinese hamster ovary cells transformed with cDNA for apoaequorin.
PubMed:	Recombinant apoaequorin acting as a pseudo-luciferase reports micromolar changes in the endoplasmic reticulum free Ca2+ of intact cells.
PubMed:	Cold-shock regulation of the Arabidopsis TCH genes and the effects of modulating intracellular calcium levels.
PubMed:	Cytosolic calcium and oxidative plant stress.
PubMed:	The moss, Physcomitrella patens, transformed with apoaequorin cDNA responds to cold shock, mechanical perturbation and pH with transient increases in cytoplasmic calcium.
PubMed:	Titration of recombinant aequorin with calcium chloride.
PubMed:	Truncation of Vargula luciferase still results in retention of luminescence.
PubMed:	Cold calcium signaling in Arabidopsis involves two cellular pools and a change in calcium signature after acclimation.
PubMed:	Differences in stability of recombinant apoaequorin within subcellular compartments.
PubMed:	Circadian oscillations of cytosolic and chloroplastic free calcium in plants.
PubMed:	Patterns of free calcium in multicellular stages of Dictyostelium expressing jellyfish apoaequorin.
PubMed:	Cause of spectral variation in the luminescence of semisynthetic aequorins.
PubMed:	Sequence of the cDNA encoding the Ca(2+)-activated photoprotein obelin from the hydroid polyp Obelia longissima.
PubMed:	Calcium waves and development.
PubMed:	Oxidative Signals in Tobacco Increase Cytosolic Calcium.
PubMed:	Changes in free calcium in the endoplasmic reticulum of living cells detected using targeted aequorin.
PubMed:	Intracellular free calcium level and its response to cAMP stimulation in developing Dictyostelium cells transformed with jellyfish apoaequorin cDNA.
PubMed:	The relative rate of aequorin regeneration from apoaequorin and coelenterazine analogues.
PubMed:	Mass spectrometric evidence for a disulfide bond in aequorin regeneration.
PubMed:	Imaging calcium dynamics in living plants using semi-synthetic recombinant aequorins.
PubMed:	Measurement of intracellular calcium using bioluminescent aequorin expressed in human cells.
PubMed:	Validity of putative calcium binding loops of photoprotein aequorin.
PubMed:	Rapid changes of mitochondrial Ca2+ revealed by specifically targeted recombinant aequorin.
PubMed:	Wind-induced plant motion immediately increases cytosolic calcium.
PubMed:	Monitoring gene expression in Chinese hamster ovary cells using secreted apoaequorin.
PubMed:	Use of recombinant biotinylated aequorin in microtiter and membrane-based assays: purification of recombinant apoaequorin from Escherichia coli.
PubMed:	A C-terminal proline is required for bioluminescence of the Ca(2+)-binding photoprotein, aequorin.
PubMed:	Transgenic plant aequorin reports the effects of touch and cold-shock and elicitors on cytoplasmic calcium.
PubMed:	Monitoring of intracellular calcium in Saccharomyces cerevisiae with an apoaequorin cDNA expression system.
PubMed:	High-level expression and purification of apoaequorin.
PubMed:	Bioluminescent immunoassay using a monomeric Fab'-photoprotein aequorin conjugate.
PubMed:	Galactose-dependent expression of the recombinant Ca2(+)-binding photoprotein aequorin in yeast.
PubMed:	Photoprotein aequorin: use as a reporter enzyme in studying gene expression in mammalian cells.
PubMed:	Bioluminescent immunoassay using a fusion protein of protein A and the photoprotein aequorin.
PubMed:	Expression and secretion of aequorin as a chimeric antibody by means of a mammalian expression vector.
PubMed:	The enzymology and molecular biology of the Ca2+-activated photoprotein, aequorin.
PubMed:	Binding of murine monoclonal antibodies to the active and inactive configurations of aequorin.
PubMed:	Overexpression and purification of the recombinant Ca2+-binding protein, apoaequorin.
PubMed:	Sequence comparisons of complementary DNAs encoding aequorin isotypes.
PubMed:	Site-specific mutagenesis of the calcium-binding photoprotein aequorin.
PubMed:	Cloning and expression of the cDNA coding for aequorin, a bioluminescent calcium-binding protein.
PubMed:	Resistivity to denaturation of the apoprotein of aequorin and reconstitution of the luminescent photoprotein from the partially denatured apoprotein.